Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana
Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana
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DOI：
10.1111/tpj.12808
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发表时间：
2015-04-01
期刊：
影响因子：
7.2
通讯作者：
Colcombet, Jean
中科院分区：
文献类型：
--
作者：
Danquah, Agyemang;de Zelicourt, Axel;Colcombet, Jean
脱落酸（阿坝）是一种重要的植物激素，参与重要的胁迫相关和植物发育过程。最近的磷酸化蛋白质组学分析显示，大量的ABA触发的磷酸化蛋白作为推定的丝裂原活化蛋白激酶（MAPK）的目标，虽然参与阿坝信号的MAPK的证据仍然很少。在这里，我们在体内鉴定并重建了一个完整的ABA激活的MAPK级联反应，由MAP 3 Ks MAP 3 K17/18、MAP 2K MKK 3和四个C组MAPK MPK 1/2/7/14组成。在植物中，我们发现mkk 3 -1和map 3 k17 map 3 k18植物中MPK 7的阿坝激活被阻断。一致地，这两个突变体表现出对阿坝的超敏性和一组ABA依赖基因的表达改变。遗传分析进一步揭示了该MAPK级联通过MAP 3 Ks的蛋白质合成被PYR/PYL/RCAR-SnRK 2-PP 2C阿坝核心信号传导模块激活，揭示了真核生物中MAPK激活的非典型机制。我们的工作提供了证据的ABA诱导的MAPK途径在plant stress signaling.Significance声明中的作用，我们在这篇文章中报告的识别一个完整的MAPK模块，由MAP 3 K17/18，MKK 3和MPK 1/2/7/14，这是激活阿坝通过阿坝核心信号复合物。我们发现，该模块的激活需要数小时的应激处理后发生的MAP 3 K蛋白合成，这表明该途径参与了阿坝和干旱的细胞反应的延迟波。
Abscisic acid (ABA) is a major phytohormone involved in important stress-related and developmental plant processes. Recent phosphoproteomic analyses revealed a large set of ABA-triggered phosphoproteins as putative mitogen-activated protein kinase (MAPK) targets, although the evidence for MAPKs involved in ABA signalling is still scarce. Here, we identified and reconstituted in vivo a complete ABA-activated MAPK cascade, composed of the MAP3Ks MAP3K17/18, the MAP2K MKK3 and the four C group MAPKs MPK1/2/7/14. In planta, we show that ABA activation of MPK7 is blocked in mkk3-1 and map3k17mapk3k18 plants. Coherently, both mutants exhibit hypersensitivity to ABA and altered expression of a set of ABA-dependent genes. A genetic analysis further reveals that this MAPK cascade is activated by the PYR/PYL/RCAR-SnRK2-PP2C ABA core signalling module through protein synthesis of the MAP3Ks, unveiling an atypical mechanism for MAPK activation in eukaryotes. Our work provides evidence for a role of an ABA-induced MAPK pathway in plant stress signalling.Significance Statement We report in this article the identification of a complete MAPK module, composed of MAP3K17/18, MKK3 and MPK1/2/7/14, which is activated by ABA through the ABA core signalling complex. We showed that the activation of this module requires the MAP3K protein synthesis which occurs after hours of stress treatment, suggesting that the pathway is involved in a delayed wave of cellular responses to ABA and drought.