Novel Sulfolobus Virus with an Exceptional Capsid Architecture
Novel Sulfolobus Virus with an Exceptional Capsid Architecture
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具有特殊衣壳结构的新型硫化叶病毒。
DOI：
10.1128/jvi.01727-17
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发表时间：
2018-03-01
影响因子：
5.4
通讯作者：
Huang, Li
中科院分区：
文献类型：
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作者：
Wang, Haina;Guo, Zhenqian;Huang, Li
从哥斯达黎加的酸性温泉中分离到一种新的古细菌病毒，命名为硫化叶菌椭圆体病毒1（SEV 1）。SEV1的病毒粒子形态独特，包含一个蛋白质衣壳，具有16个规则间隔的条纹和一个11 nm厚的包膜。衣壳表现出一种不寻常的结构，其中病毒DNA，可能是核蛋白丝的形式，在一个平面上缠绕在病毒体的纵轴上，形成一个有中心孔的多层盘状结构，其中16个结构堆叠在一起，产生一个线轴状衣壳。SEV1基因组为线性双链DNA，长度约23 kb，编码38个开放阅读框（ORF）。在少数具有推定功能的ORF中，有一个编码蛋白质引发的DNA聚合酶的基因。SEV 1感染的细胞表面出现六重对称的病毒相关金字塔（VAP），这些金字塔破裂，形成六边形开口，随后释放后代病毒颗粒。值得注意的是，SEV1病毒体在宿主细胞的细胞质中获得脂质膜。病毒包膜的脂质组成与细胞膜的脂质组成相关。这些结果表明，使用一个独特的机制SEV1在膜biogenesy.IMPORTANCE古细菌病毒的调查，大大扩大了我们的知识，病毒球及其在生命进化中的作用。在这里，我们表明，硫化叶菌椭圆体病毒1（SEV1），从哥斯达黎加的温泉分离的古细菌病毒，表现出一种新的病毒形状和不寻常的衣壳结构。SEV1 DNA在围绕病毒体纵轴的平面内缠绕多次以形成盘状结构，并且这些结构中的16个堆叠以产生线轴状衣壳。病毒在细胞内获得其包膜，并通过在宿主细胞表面上产生六边形孔而离开宿主细胞。这些结果揭示了病毒形态发生的多样性。
A novel archaeal virus, denoted Sulfolobus ellipsoid virus 1 (SEV1), was isolated from an acidic hot spring in Costa Rica. The morphologically unique virion of SEV1 contains a protein capsid with 16 regularly spaced striations and an 11-nm-thick envelope. The capsid exhibits an unusual architecture in which the viral DNA, probably in the form of a nucleoprotein filament, wraps around the longitudinal axis of the virion in a plane to form a multilayered disk-like structure with a central hole, and 16 of these structures are stacked to generate a spool-like capsid. SEV1 harbors a linear double-stranded DNA genome of similar to 23 kb, which encodes 38 predicted open reading frames (ORFs). Among the few ORFs with a putative function is a gene encoding a protein-primed DNA polymerase. Sixfold symmetrical virus-associated pyramids (VAPs) appear on the surface of the SEV1-infected cells, which are ruptured to allow the formation of a hexagonal opening and subsequent release of the progeny virus particles. Notably, the SEV1 virions acquire the lipid membrane in the cytoplasm of the host cell. The lipid composition of the viral envelope correlates with that of the cell membrane. These results suggest the use of a unique mechanism by SEV1 in membrane biogenesis.IMPORTANCE Investigation of archaeal viruses has greatly expanded our knowledge of the virosphere and its role in the evolution of life. Here we show that Sulfolobus ellipsoid virus 1 (SEV1), an archaeal virus isolated from a hot spring in Costa Rica, exhibits a novel viral shape and an unusual capsid architecture. The SEV1 DNA wraps multiple times in a plane around the longitudinal axis of the virion to form a disk-like structure, and 16 of these structures are stacked to generate a spool-like capsid. The virus acquires its envelope intracellularly and exits the host cell by creating a hexagonal hole on the host cell surface. These results shed significant light on the diversity of viral morphogenesis.