Orderly Osteochondral Regeneration in a Sheep Model Using a Novel Nano-Composite Multilayered Biomaterial
Orderly Osteochondral Regeneration in a Sheep Model Using a Novel Nano-Composite Multilayered Biomaterial
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DOI：
10.1002/jor.20958
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Marcacci, Maurillo
中科院分区：
文献类型：
--
作者：
Kon, Elizaveta;Delcogliano, Marco;Marcacci, Maurillo
本文的目的是在动物模型中研究一种新开发的仿生支架用于骨软骨缺损的安全性和再生潜力。用羟基磷灰石纳米颗粒将胶原原纤维成核，制备了一种新型的多层梯度纳米复合支架。在12只羊的股骨髁中，形成了24个骨软骨病变。动物随机分为三个治疗组：支架单独治疗组、体外定植自体软骨细胞支架组和空缺损组。术后6个月，处死动物，对病变进行组织学评估。标本的组织学和大体评价显示，除对照组外，所有组的软骨表面都融合良好。单独接受支架组和负载自体软骨细胞支架组的骨再生均有明显改善。细胞植入组和无细胞组在软骨表面重建和骨软骨缺损填充方面无显著差异。对照组无骨、软骨缺损愈合，缺损内填充纤维组织。定量宏观和组织学评分评价证实了所观察到的定性趋势。本研究结果表明，这种新型骨软骨支架安全且易于使用，可能是指导和协调骨和透明样软骨再生过程的合适基质。有或没有自体软骨细胞的可比较的再生过程表明，支架的主要作用方式是基于局部细胞的募集。(C) 2009骨科研究学会。Wiley期刊公司出版。[J]中华骨科杂志，2010
The objective of this article was to investigate the safety and regenerative potential of a newly developed biomimetic scaffold when applied to osteochondral defects in an animal model. A new multilayer gradient nano-composite scaffold was obtained by nucleating collagen fibrils with hydroxyapatite nanoparticles. In the femoral condyles of 12 sheep, 24 osteochondral lesions were created. Animals were randomized into three treatment groups: scaffold alone, scaffold colonized in vitro with autologous chondrocytes and empty defects. Six months after surgery, the animals were sacrificed and the lesions were histologically evaluated. Histologic and gross evaluation of specimens showed good integration of the chondral surface in all groups except for the control group. Significantly better bone regeneration was observed both in the group receiving the scaffold alone and in the group with scaffold loaded with autologous chondrocytes. No difference in cartilage surface reconstruction and osteochondral defect filling was noted between cell-seeded and cell-free groups. In the control group, no bone or cartilage defect healing occurred, and the defects were filled with fibrous tissue. Quantitative macroscopic and histological score evaluations confirmed the qualitative trends observed. The results of the present study showed that this novel osteochondral scaffold is safe and easy to use, and may represent a suitable matrix to direct and coordinate the process of bone and hyaline-like cartilage regeneration. The comparable regeneration process observed with or without autologous chondrocytes suggests that the main mode of action of the scaffold is based on the recruitment of local cells. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:116-124, 2010