Relationship between moiré patterns, tubulin shape, and microtubule polarity.

Relationship between moiré patterns, tubulin shape, and microtubule polarity.
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莫尔图案、微管蛋白形状和微管极性之间的关系。

DOI:
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发表时间:
1998
影响因子:
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通讯作者:
D. Chrétien
D. Chrétien
中科院分区:
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文献类型:
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作者:
H. Sosa;D. Chrétien

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微管的极性反映在冷冻电子显微镜图像和三维重建中[Chrétien et al.,1996:Structure 4:1031-1040; Sosa和Milligan,1996:J. Mol. Biol. 260:743-755]。本文展示了波纹图案的方向性和微管蛋白亚基的形状如何相关。冷冻电子显微镜观察到的微管显示出箭头莫尔图案,该图案指向具有右手原丝偏斜的微管的正端,并指向具有左手原丝偏斜的微管的负端。另一方面,从正端观察到的微管的三维重建揭示了微管蛋白亚基的径向逆时针回转。我们展示了这些极性特征是如何相关的,并提出了明确的规则,用于确定微管的纵向和轴向视图上的极性。
The polarity of microtubules is reflected in cryo-electron microscope images and in three-dimensional reconstructions [Chrétien et al., 1996: Structure 4:1031-1040; Sosa and Milligan, 1996: J. Mol. Biol. 260:743-755]. This paper shows how the directionality of the moiré patterns and the shape of the tubulin subunits are related. Microtubules observed by cryo-electron microscopy show an arrowhead moiré pattern that points toward the plus end of microtubules with a right-handed protofilament skew and toward the minus end of microtubules with a left-handed protofilament skew. On the other hand, three-dimensional reconstructions of microtubules observed from the plus end reveal a radial counterclockwise slew of the tubulin subunits. We show how these polar features are related and present unambiguous rules for determining the polarity on longitudinal and axial views of microtubules.
DOI: 10.1006/jmbi.1996.0434
发表时间: 1996
影响因子: 5.6
作者:
Sosa,H;Milligan,RA
通讯作者: Milligan,RA
DOI: 10.1006/jsbi.1997.3851
发表时间: 1997
影响因子: 3
作者:
Sosa,H;Hoenger,A;Milligan,RA
通讯作者: Milligan,RA