The metabolic regulation of fenofibrate is dependent on dietary protein content in male juveniles of Nile tilapia (Oreochromis niloticus)
The metabolic regulation of fenofibrate is dependent on dietary protein content in male juveniles of Nile tilapia (Oreochromis niloticus)
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非诺贝特的代谢调节取决于尼罗罗非鱼（Oreochromis niloticus）雄性幼鱼的膳食蛋白质含量
DOI：
10.1017/s0007114519001594
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发表时间：
2019
影响因子：
3.6
通讯作者：
Du Z-Y
中科院分区：
文献类型：
--
作者：
Ning L.;Liu Y.;Wang W.;Li Y.;Chen L.;Du Z-Y
非诺贝特在哺乳动物体内的作用受到多种因素的影响，如膳食营养水平和生理状态。然而，这种现象在鱼类中尚未得到很好的研究。本研究旨在探讨饲料蛋白质含量对尼罗罗非鱼非诺贝特代谢调控的影响。进行了为期8周的实验，用两种蛋白质水平（28%和38%）的四种饲料喂鱼，添加或不添加非诺贝特（200 mg/kg体重/d）。试验结束后，对试验动物的身体形态学指标、血浆生化指标和定量PCR数据进行检测。这些结果表明，非诺贝特显著降低低蛋白（LP）饲养的鱼的摄食量和增重率，增加氧化应激（增加血浆甲烷二羧酸）和肝体比（增加肝体指数）。与此相反，非诺贝特表现出降脂（降低肝脂）的效果，上调基因的表达与脂质catalysts，运输和合成代谢的高蛋白（HP）喂养的鱼。本研究提示，高能量HP饲料可增强非诺贝特的降脂作用，而低能量LP饲料则会对非诺贝特的代谢产生不利影响。总之，我们的研究表明，非诺贝特在尼罗罗非鱼的代谢调节不仅取决于饲料能量含量，而且还取决于膳食营养成分，如膳食蛋白质和/或脂质水平。
The fenofibrate functions in mammals could be affected by many factors such as dietary nutrient levels and physiological status. However, this phenomenon has not been well studied in fish. The goal of our study was to investigate the effect of dietary protein contents on metabolic regulation of fenofibrate in Nile tilapia. An 8-week experiment was conducted to feed fish with four diets at two protein levels (28 and 38 %) with or without the supplementation of fenofibrate (200 mg/kg body weight per d). After the trial, the body morphometric parameters, plasma biochemical parameters and quantitative PCR data were examined. These results showed that fenofibrate significantly reduced the feeding intake and weight gain rate, increased the oxidative stress (increased plasma methane dicarboxylic aldehyde) and liver : body ratio (increased hepatosomatic index) in the low protein (LP)-fed fish. In contrast, fenofibrate exhibited a lipid-lowering (reduced hepatic lipid) effect and up-regulated the expressions of the genes related to lipid catabolism, transport and anabolic metabolism in the high protein (HP)-fed fish. The present study suggested that lipid-lowering effect of fenofibrate would be strengthened in the fish fed with the HP diet containing high energy, but in the fish fed with the LP diet containing low energy, the fenofibrate treatment would cause adverse effects for metabolism. Taking together, our study showed that the metabolic regulation of fenofibrate in Nile tilapia was dependent not only on feed energy content but also on dietary nutrient composition, such as dietary protein and/or lipid levels.