A Lead(II)-Driven DNA Molecular Device for Turn-On Fluorescence Detection of Lead(II) Ion with High Selectivity and Sensitivity
A Lead(II)-Driven DNA Molecular Device for Turn-On Fluorescence Detection of Lead(II) Ion with High Selectivity and Sensitivity
复制标题
一种铅(II)驱动的DNA分子装置，用于高选择性和高灵敏度铅(II)离子的开启荧光检测
DOI：
10.1021/ja105849m
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发表时间：
2010-09-29
影响因子：
15
通讯作者：
Wang, Erkang
中科院分区：
文献类型：
--
作者：
Li, Tao;Dong, Shaojun;Wang, Erkang
利用基于DNA双链四链交换的Pb2+驱动DNA分子器件，实现了对Pb2+的高选择性和高灵敏度的检测。这种DNA装置的能量来源于Pb2+稳定G-四链的出色效率，使DNA双链解离从而驱动该装置。通过添加强的Pb2+螯合剂DOTA，可使该设备恢复到原来的状态，使设备具有良好的可重用性。在整个过程中，信号读出是通过与G-四链结合并从G-四链释放的荧光探针来调制的。这种DNA器件可以作为一种新型的开启式荧光传感器，用于Pb2+的高选择性和高灵敏度的检测。
A Pb2+-driven DNA molecular device which is constructed based on a DNA duplex quadruplex exchange is utilized for the highly selective and sensitive detection of Pb2+. The power of this DNA device originates from the excellent efficiency of Pb2+ for stabilizing G-quadruplexes, which makes the DNA duplex unwind thereby driving the device. This device can be reset to the original state by addition of a strong Pb2+ chelator DOTA, endowing the device with good reusability. In the whole process, the signal readout is modulated via a fluorescent probe binding to and being released from the G-quadruplex. Such a DNA device can serve as a novel turn-on fluorescent sensor for Pb2+ detection with high selectivity and sensitivity.