Prostaglandin F2a-PTGFR signalling activation, growth factor expression and cell proliferation in bovine endometrial explants
Prostaglandin F2a-PTGFR signalling activation, growth factor expression and cell proliferation in bovine endometrial explants
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牛子宫内膜外植体中前列腺素 F2a-PTGFR 信号激活、生长因子表达和细胞增殖
DOI：
10.1071/rd16144
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发表时间：
2017
影响因子：
1.9
通讯作者：
Cao Jinshan
中科院分区：
文献类型：
--
作者：
Zhang Shuangyi;Liu Bo;Gao Long;Mao Wei;Fu Changqi;Duritahala;Zhang Nan;Zhang Ying;Shen Yuan;Cao Jinshan
家畜的子宫内膜经历了定期的再生和退化，并在发情周期中表现出显著的自我修复能力。在着床期和早孕期间也可以观察到子宫内膜的生长模式，但这些过程中子宫内膜生长的机制尚不清楚。在这些过程中，子宫内膜的生长与前列腺素(PG)F2α的分泌呈正相关，但PgF2α在子宫内膜生长中的作用研究较少。本研究采用体外培养的牛子宫内膜组织，研究了子宫内膜细胞增殖及一系列促子宫内膜生长的生长因子对前列腺素F_2受体(α受体)激活的影响。实时定量逆转录聚合酶链式反应和Western blotting结果显示，加入氟前列醇(10β9~10 5 M)后，牛子宫内膜组织中结缔组织生长因子、成纤维细胞生长因子2、白介素8、基质金属蛋白酶2、转化生长因子1和血管内皮生长因子A的基因和蛋白表达增加(P−<；−0.05)，并诱导细胞增殖，包括上皮和成纤维细胞增殖。而氟前列烯醇(10−9~10−5M，P <； )处理后，caspase-3蛋白表达降低。这些结果可能有助于确定PGF2PTGFR信号通路在子宫内膜生长中可能发挥的作用。
The endometrium of domestic animals undergoes regular periods of regeneration and degeneration and exhibits a remarkable capacity for self-repair during the oestrous cycle. The endometrial growth pattern is also observed during in the implantation period and early pregnancy, but the mechanism underlying endometrial growth in these processes remains unclear. A positive correlation between endometrial growth in these processes and prostaglandin (PG) F2α secretion has been reported, but the roles that PGF2α plays in endometrial growth is less studied. In the present study, cell proliferation and the responses of a series of growth factors essential for endometrial growth to PGF2α receptor (PTGFR) activation were investigated in bovine endometrial explants in vitro. Using real-time reverse transcription–polymerase chain reaction and western blotting, mRNA and protein expression of connective tissue growth factor, fibroblast growth factor2, interleukin8, matrix metalloproteinase2, transforming growth factor β1 and vascular endothelial growth factor A was increased (P < 0.05) and cell proliferation, including epithelial and fibroblast proliferation, was induced in response to increased levels of proliferating cell nuclear antigen, cytokeratin-18 and fibroblast-specific protein-1 (P < 0.05) following PTGFR activation by adding fluprostenol (10−9–10−5 M) into culture medium of bovine endometrial explants. However, caspase-3 protein expression was reduced following treatment of explants with fluprostenol (10−9–10−5 M, P < 0.05). These results may help define the possible roles the PGF2α–PTGFR signalling pathway plays in endometrial growth.