Comparison of the chicken and zebra finch Z chromosomes shows evolutionary rearrangements
Comparison of the chicken and zebra finch Z chromosomes shows evolutionary rearrangements
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DOI：
10.1007/s10577-006-1082-1
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发表时间：
2006-12-01
影响因子：
2.6
通讯作者：
Arnold, Arthur P.
中科院分区：
文献类型：
--
作者：
Itoh, Yuichiro;Kampf, Kathy;Arnold, Arthur P.
采用荧光原位杂交（FISH）技术对鸡斑马雀（Taeniopygia guttata）细菌人工染色体（BAC）克隆的14个z连锁基因ATP5A1、CHD1、NR2F1、DMRT1、PAM、GHR、HSD17B4、NIPBL、ACO1、HINT1、SMAD2、SPIN、NTRK2和UBE2R2进行了染色体定位。所有14个基因也都映射到斑胸草雀的Z染色体上，这表明在两个鸟类谱系中Z染色体上的基因含量存在实质性的保护。然而，斑胸草雀Z染色体上这些基因的物理顺序与鸡的不同，由于这两个谱系分化，这种模式需要多次反转。14个斑胸草雀BAC DNA中有8个与W染色体交叉杂交，通常与整个W染色体交叉杂交，表明W染色体和Z染色体共享重复序列。这些重复的序列可能是在雀系从雁形谱系分化出来后进化而来的。
Using fluorescent in-situ hybridization (FISH) of zebra finch (Taeniopygia guttata) bacterial artificial chromosome (BAC) clones, we determined the chromosomal localizations of 14 zebra finch genes that are Z-linked in chickens: ATP5A1, CHD1, NR2F1, DMRT1, PAM, GHR, HSD17B4, NIPBL, ACO1, HINT1, SMAD2, SPIN, NTRK2 and UBE2R2. All 14 genes also map to the zebra finch Z chromosome, indicating substantial conservation of gene content on the Z chromosome in the two avian lineages. However, the physical order of these genes on the zebra finch Z chromosome differed from that of the chicken, in a pattern that would have required several inversions since the two lineages diverged. Eight of 14 zebra finch BAC DNA showed cross-hybridization to the W chromosome, usually to the entire W chromosome, suggesting that repetitive sequences are shared by the W and Z chromosomes. These repetitive sequences likely evolved in the finch lineage after it diverged from the Galliform lineage.