Location, transcription, and sequence of a baculovirus gene encoding a small arginine-rich polypeptide
Location, transcription, and sequence of a baculovirus gene encoding a small arginine-rich polypeptide
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DOI：
10.1128/jvi.61.3.661-666.1987
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发表时间：
1987-03
影响因子：
5.4
通讯作者：
M. E. Wilson;T. Mainprize;P. Friesen;Lois K. MILLERt
中科院分区：
文献类型：
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作者：
M. E. Wilson;T. Mainprize;P. Friesen;Lois K. MILLERt
对加州自签名核多角体病毒丰富的0.5千碱基后期转录物cDNA的核苷酸序列分析显示，一个小的开放阅读框编码一个富含精氨酸的6.9千道尔顿蛋白。预测的6.9千道尔顿蛋白的氨基酸组成与病毒核衣壳核心蛋白的氨基酸组成基本相同。通过S1核酸酶和引物延伸分析，确定了转录本5‘和3’端的精确位置。通过该区域的多个重叠转录物包括三个早期RNA和三个丰富的晚期RNA，它们逆时针转录，一个瞬时RNA顺时针转录，相对于病毒的物理图谱。
The nucleotide sequence of the cDNA of an abundant late 0.5-kilobase transcript of Autographa californica nuclear polyhedrosis virus revealed a small open reading frame encoding an arginine-rich 6.9-kilodalton protein. The predicted amino acid composition of the 6.9-kilodalton protein was essentially identical to that of the core protein of viral nucleocapsids. The precise location of the 5' and 3' ends of the transcript were confirmed by S1 nuclease and primer extension analyses. Multiple overlapping transcripts through this region include three early and three abundant late RNAs which are transcribed counterclockwise and one transient RNA which is transcribed clockwise with respect to the physical map of the virus.