LISTERIA-MONOCYTOGENES INTRAGASTRIC AND INTRAPERITONEAL APPROXIMATE 50-PERCENT LETHAL DOSES FOR MICE ARE COMPARABLE, BUT DEATH OCCURS EARLIER BY INTRAGASTRIC FEEDING
LISTERIA-MONOCYTOGENES INTRAGASTRIC AND INTRAPERITONEAL APPROXIMATE 50-PERCENT LETHAL DOSES FOR MICE ARE COMPARABLE, BUT DEATH OCCURS EARLIER BY INTRAGASTRIC FEEDING
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DOI：
10.1128/iai.58.9.2940-2945.1990
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发表时间：
1990-09-01
影响因子：
3.1
通讯作者：
PLIKAYTIS, BD
中科院分区：
文献类型：
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作者：
PINE, L;MALCOLM, GB;PLIKAYTIS, BD
腹膜腔(I.P.)和胃内(I.G.)测定了15株食品和临床分离的单核细胞增多性李斯特菌的小鼠近似半数致死剂量值(ALD50)。尽管所有菌株都能产生免疫球蛋白。ALD50相当于或低于他们的IP。ALD50S，I.G.在为期6天的试验的前3天，大多数菌株的喂养导致的死亡人数比ip更多。注射。ALD50为50~4.4倍。105个单元，具有大约1-对数95%的置信度区间。在通过牛奶悬浮液或牛奶生长测试的五种菌株中，没有一种能产生I.G.ALD50低于洗涤细胞。结果10到21克的小鼠支持使用15只小鼠进行igg。试验：21-g小鼠对igg更具抵抗力。感染。这些结果表明，I.G.饲喂允许评估载体(如牛奶)在确定李斯特菌毒力中的作用，允许通过I.P.对菌株进行表征。和I.G.ALD50，并强调当细菌被引入I.G.时，可能会更快地感染。
The intraperitoneal (i.p.) and intragastric (i.g.) mouse approximate 50% lethal dose values (ALD50s) were determined for 15 food and clinical isolates of Listeria monocytogenes. Although all strains gave i.g. ALD50S comparable to or less than their i.p. ALD50S, the i.g. feeding of most strains produced more deaths within the first 3 days of the 6-day test than did i.p. injection. ALD50S ranged from 50 to 4.4 .times. 105 cells with approximately 1-log 95% confidence intervals. Of five strains tested by suspension in milk or by growth in milk, none gave i.g. ALD50S that were lower than those of washed cells. Results with 10- to 21-g mice supported the use of 15-mice for i.g. testing; 21-g mice were more resistant to i.g. infection. These results indicate that i.g. feeding permits an evaluation of the role of the carrier (such as milk) in the determination of listerial virulence, permits strain characterization by i.p. and i.g. ALD50s, and emphasizes a potentially more rapid infection when the bacterium is introduced i.g.