Gene expression and localization of two types of AQP5 in Xenopus tropicalis under hydration and dehydration.
Gene expression and localization of two types of AQP5 in Xenopus tropicalis under hydration and dehydration.
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DOI：
10.1152/ajpregu.00186.2013
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发表时间：
2014-07
期刊：
影响因子：
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通讯作者：
Y. Shibata;T. Sano;Nobuhito Tsuchiya;R. Okada;H. Mochida;Shigeyasu Tanaka;Masakazu Suzuki
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文献类型：
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作者：
Y. Shibata;T. Sano;Nobuhito Tsuchiya;R. Okada;H. Mochida;Shigeyasu Tanaka;Masakazu Suzuki
通过同源性比较和分子系统发育分析，在热带爪蟾基因组中鉴定出aqp-xt5a和aqp-xt5b两种水通道蛋白5 （AQP5）基因。当青蛙在水中时，AQP-xt5a mRNA在皮肤和膀胱中表达。脱水青蛙AQP-xt5a mRNA表达显著升高。皮肤中也检测到AQP-xt5b mRNA，并在脱水反应中升高。脱水后肺和胃AQP-xt5b mRNA开始轻微表达。对于水化蛙盆腔皮肤，免疫荧光染色将AQP-xt5a定位在颗粒腺分泌细胞的细胞质上，AQP-xt5b定位在小颗粒腺分泌细胞的顶质膜上。脱水后，两种aqp在各自腺体中的位置没有变化，但AQP-xt5a在小颗粒腺体分泌细胞的细胞质中可见。对于膀胱，在正常水合作用下，在一些颗粒细胞的顶质膜和细胞质中观察到AQP-xt5a。脱水后，AQP-xt5a存在于大多数颗粒细胞的顶膜和细胞质中。然而，在水合青蛙体内注射缩宫素后，AQP-xt5a在小颗粒腺体和膀胱中的定位并未发生变化。提示AQP-xt5a可能参与脱水时膀胱水分的再吸收，而AQP-xt5b可能参与小颗粒腺的水分分泌。
Two types of aquaporin 5 (AQP5) genes (aqp-xt5a and aqp-xt5b) were identified in the genome of Xenopus tropicalis by synteny comparison and molecular phylogenetic analysis. When the frogs were in water, AQP-xt5a mRNA was expressed in the skin and urinary bladder. The expression of AQP-xt5a mRNA was significantly increased in dehydrated frogs. AQP-xt5b mRNA was also detected in the skin and increased in response to dehydration. Additionally, AQP-xt5b mRNA began to be slightly expressed in the lung and stomach after dehydration. For the pelvic skin of hydrated frogs, immunofluorescence staining localized AQP-xt5a and AQP-xt5b to the cytoplasm of secretory cells of the granular glands and the apical plasma membrane of secretory cells of the small granular glands, respectively. After dehydration, the locations of both AQPs in their respective glands did not change, but AQP-xt5a was visualized in the cytoplasm of secretory cells of the small granular glands. For the urinary bladder, AQP-xt5a was observed in the apical plasma membrane and cytoplasm of a number of granular cells under normal hydration. After dehydration, AQP-xt5a was found in the apical membrane and cytoplasm of most granular cells. Injection of vasotocin into hydrated frogs did not induce these changes in the localization of AQP-xt5a in the small granular glands and urinary bladder, however. The results suggest that AQP-xt5a might be involved in water reabsorption from the urinary bladder during dehydration, whereas AQP-xt5b might play a role in water secretion from the small granular gland.