Synthesis of tropomyosin in myogenic cultures and in RNA-directed cell-free systems: Qualitative changes in the polypeptides
Synthesis of tropomyosin in myogenic cultures and in RNA-directed cell-free systems: Qualitative changes in the polypeptides
复制标题
生肌培养物和 RNA 指导的无细胞系统中原肌球蛋白的合成：多肽的质变
DOI：
10.1016/0092-8674(78)90124-1
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发表时间：
1978
期刊：
影响因子：
64.5
通讯作者：
D. Yaffe
中科院分区：
文献类型：
--
作者：
Y. Carmon;S. Neuman;D. Yaffe
在分化培养的成肌细胞系和在小麦胚芽无细胞系统中，由在分化的不同阶段提取的纯化RNA指导，研究了具有β-和β-原肌球蛋白性质的多肽的合成。在等电聚焦电泳、SDS双向凝胶电泳和SDS-尿素/SDS双向凝胶电泳中，多肽与原肌球蛋白共迁移。与真正的原肌球蛋白一样，这些多肽在尿素存在下会极大地改变其流动性，并且不会被脯氨酸标记。在完整细胞和无细胞系统中合成的p原肌球蛋白可以通过等电聚焦分离成至少两种组分。一种组分（指定PI）在所有发育阶段都存在少量，而一种更基本的组分（&）对分化培养物具有特异性。在完整细胞中的合成和纯化的RNA在无细胞系统中指导其合成的能力变得可检测，并且在单核细胞融合成多核纤维期间大大增加。结果表明，p1和β原肌球蛋白是由不同的基因编码的。
The synthesis of polypeptides with the properties of (Y and p tropomyosin was investigated in differentiating cultures of a myogenic cell line and in a wheat germ cell-free system directed by purified RNA extracted at different stages of differentiation. The polypeptides co-migrate with tropomyosin in isoelectric focusing and SDS two-dimensional gel electrophoresis and SDS-urea/SDS two-dimensional gels. Like authentic tropomyosin, these polypeptides change their mobility greatly in the presence of urea and do not become labeled with proline. The p tropomyosin synthesized in the intact cells and in the cell-free system can be separated by isoelectric focusing into at least two components. One component(designated PI) is present in a small amount at all developmental stages examined, and a more basic component(&) is specific for differentiated cultures. The synthesis of & in the intact cells and the capacity of purified RNA to direct its synthesis in a cell-free system become detectable and increase greatly during the period of fusion of the mononucleated cells into multinucleated fibers. The results suggest that the p1 and & tropomyosins are coded for by different genes.