Taenia crassiceps cysticercosis:: immune response in susceptible and resistant BALB/c mouse substrains
Taenia crassiceps cysticercosis:: immune response in susceptible and resistant BALB/c mouse substrains
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DOI：
10.1007/s00436-003-0848-z
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发表时间：
2003-06-01
影响因子：
2
通讯作者：
Sciutto, E
中科院分区：
文献类型：
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作者：
López-Briones, S;Lamoyi, E;Sciutto, E
粗头绦虫可以自然地和实验性地感染啮齿动物，它们通过出芽繁殖。对T.在BALB/c的两个亚株：BALB/cAnN（敏感）和BALB/cJ（抗性）之间发现了粗头囊虫病。在嵌合体小鼠中，抗性被转移到具有来自抗性小鼠的骨髓细胞的易感小鼠，这支持抗性表型的免疫介导。为了进一步探索可能导致这些易感性差异的免疫应答，在两种亚株中探索了寄生虫引起的特异性细胞免疫应答。仅在抗性小鼠的脾细胞中，囊尾蚴抗原诱导增殖反应和IL-2水平增加。BALB/cAnN小鼠脾细胞CD 4（+）、CD 8（+）细胞百分率分别下降11.1%、17.5%。一项TCRV β库研究显示，仅在易感BALB/cAnN品系中，CD 4（+）和CD 8（+）脾细胞中的V β 2显著降低。
Taenia crassiceps can naturally and experimentally infect rodents in which they reproduce by budding. Differences in the susceptibility to T. crassiceps cysticercosis were found between two BALB/c substrains: BALB/cAnN (susceptible) and BALB/cJ (resistant). In chimeric mice, resistance was transferred to susceptible mice with bone marrow cells from the resistant mice, which argues in favor of an immune mediation of the resistant phenotype. To further explore the immune response that could underlie these differences in susceptibility, the specific cellular immune response elicited by the parasite was explored in both substrains. An increased proliferative response and IL-2 levels were induced by cysticercal antigens only in splenocytes from resistant mice. A decrease in the percentage of CD4(+) (11.1%), CD8(+) (17.5%) was found in splenocytes from susceptible BALB/cAnN mice. A study of the TCRVbeta repertoire revealed a significant decrease in Vbeta2 in both CD4(+) and CD8(+) splenocytes only in the susceptible BALB/cAnN strain.