Investigation of the structural requirements in the lipopolysaccharide core acceptor for ligation of O antigens in the genus Salmonella -: WaaL "ligase" is not the sole determinant of acceptor specificity
Investigation of the structural requirements in the lipopolysaccharide core acceptor for ligation of O antigens in the genus Salmonella -: WaaL "ligase" is not the sole determinant of acceptor specificity
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DOI：
10.1074/jbc.m401366200
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发表时间：
2004-08-27
影响因子：
4.8
通讯作者：
Whitfield, C
中科院分区：
文献类型：
--
作者：
Kaniuk, NA;Vinogradov, E;Whitfield, C
O抗原多糖与脂类A核心寡糖的连接是脂多糖形成复杂糖脂的后期步骤。虽然这一过程已经局限于内膜的周质表面，但其连接机制的细节仍未得到解决。到目前为止，只有一种已知的基因产物(Waal，通常被称为“连接酶”)是必需的。连接活性需要特定的脂类A核心低聚糖受体结构，有人认为Waal蛋白赋予这种受体特异性。在这里，我们研究了肠炎沙门氏菌原型血清型O抗原连接的核心低聚糖受体的结构要求。在具有特定核心低聚糖结构的突变体中的互补实验表明，针对特定核心低聚糖结构的连接反应的特异性并不仅仅依赖于Waal蛋白。这些数据提供了涉及更多细胞成分的更复杂识别过程的第一个迹象。
The ligation of O antigen polysaccharide to lipid A-core oligosaccharide is a late step in the formation of the complex glycolipid known as lipopolysaccharide. Although the process has been localized to the periplasmic face of the inner membrane, details of the ligation mechanism have not been resolved. To date, there is only one gene product (WaaL, often referred to as "ligase") known to be required. There exists a requirement for a specific lipid A-core oligosaccharide acceptor structure for ligation activity, and it has been proposed that the WaaL protein imparts this acceptor specificity. Here the structural requirements in the core oligosaccharide acceptor for O antigen ligation are investigated in prototype serovars of Salmonella enterica. Complementation experiments in mutants with defined core oligosaccharide structure indicate that the specificity of the ligation reaction for a particular core oligosaccharide structure is not dependent on the WaaL protein alone. The data provide the first indication of a more complicated recognition process involving additional cellular components.