Colocalization of P450 Aromatase and Oxytocin Immunostaining in the Rat Hypothalamus
Colocalization of P450 Aromatase and Oxytocin Immunostaining in the Rat Hypothalamus
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DOI：
10.1055/s-0032-1327680
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发表时间：
2013-04-01
影响因子：
2.2
通讯作者：
Jirikowski, G. F.
中科院分区：
文献类型：
--
作者：
Dief, A. El-Emam;Caldwell, J. D.;Jirikowski, G. F.
结合免疫过氧化物酶和免疫荧光，我们观察到共定位的细胞色素P450芳香化酶与后叶肽催产素及其相关的neurophysin 1在成年雄性大鼠。P450在下丘脑前部最丰富。OT与P450共定位于视前区、下丘脑室周围核、外侧连合下核和齿状回。在视上核和室旁核的巨细胞胞体仅偶尔含有这两种抗原。P450免疫染色与已知的雌激素靶区有很大程度的重叠。催产素能功能由雌二醇控制，而下丘脑神经内分泌核团中大多数缺乏雄激素受体。我们的研究结果表明，系统的雄激素可能芳香化的雌激素在男性催产素能神经元连接到边缘系统。
With combined immunoperoxidase and immunofluorescence, we observed colocalization of cytochrome P450 aromatase with the posterior lobe peptide oxytocin and its associated neurophysin 1 in adult male rats. P450 was most abundant in the anterior hypothalamus. Colocalization of OT with P450 was observed in the preoptic region, the periventricular nucleus of the hypothalamus, the lateral subcommissural nucleus, and in the zona incerta. Magnocellular perikarya in the supraoptic and in the paraventricular nuclei contained only occasionally both antigens. P450 immunostaining overlapped to a great extent with known estrogen target regions. Oxytocinergic functions are controlled by estradiol while androgen receptors are mostly absent in neuroendocrine hypothalamic nuclei. Our findings suggest that systemic androgens may be aromatized to estrogens in male oxytocinergic neurons linked to the limbic system.