Accumulation of alpha 2u-globulin in the renal proximal tubules of male rats exposed to unleaded gasoline.
Accumulation of alpha 2u-globulin in the renal proximal tubules of male rats exposed to unleaded gasoline.
复制标题
暴露于无铅汽油的雄性大鼠肾近端小管中α2u-球蛋白的积累。
DOI：
10.1016/0041-008x(87)90304-8
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发表时间：
1987
影响因子：
3.8
通讯作者：
Roy,AK
中科院分区：
文献类型：
--
作者：
Olson,MJ;Garg,BD;Murty,CV;Roy,AK
在发动机燃料中发现的饱和支链脂肪烃会对雄性大鼠产生肾毒性。用无铅汽油（0.04- 2.0ml/kg体重，po）处理雄性大鼠9天，在高剂量水平下，肾近曲小管（PCT）上皮细胞中透明（蛋白质再吸收）液滴的数量和大小明显增加，细胞脱落也明显增强。在肾小球、远端小管或髓质中未观察到其他给药相关病理学效应。给予汽油（1.0 ml/kg，po，9天）后，雄性大鼠的主要尿蛋白α 2u-球蛋白的肾脏含量最大增加约4.4倍;在更高剂量下未观察到进一步增加。肾组织切片的α 2u-球蛋白免疫过氧化物酶染色显示，许多PCT上皮细胞中存在大量抗原积聚，其中含有透明小滴。汽油对肝脏α 2u-球蛋白及其mRNA含量无明显影响。这些数据首次表明，α 2u-球蛋白在汽油中毒雄性大鼠的肾脏中蓄积，并特异性地被隔离在汽油诱导的肾病的某些透明液滴中。碳氢化合物诱导的肾溶酶体降解低分子量尿蛋白的缺陷，而不是这些蛋白质的合成增加，似乎导致透明小滴积聚。
Saturated branched-chain aliphatic hydrocarbons, found in motor fuels, induce nephrotoxicity in male rats. Treatment of male rats with unleaded gasoline (0.04–2.0 ml/kg body wt, po) for 9 days increased markedly the number and size of hyaline (protein resorption) droplets in epithelial cells of the renal proximal convoluted tubules (PCT) and enhanced cellular exfoliation at high dose levels. No other treatment-related pathological effects were observed in the plomeruli, distal tubules, or medulla. The renal content of α2u-globulin, a major urinary protein of male rats, was increased maximally by about 4.4-fold after gasoline administration (1.0 ml/kg, po, 9 days); no further increase was observed at higher doses. Immunoperoxidase staining of kidney tissue sections for α2u-globulin revealed large accumulations of antigen localized in many of the PCT epithelial cells which contained hyaline droplets. The hepatic content of α2u-globulin and its mRNA were not altered by gasoline administration. These data show, for the first time, that α2u-globulin is accumulated in the kidneys of gasoline-intoxicated male rats and sequestered specifically in some of the hyaline droplets characteristic of gasoline-induced nephropathy. A hydrocarbon-induced defect in the renal lysosomal degradation of low-molecular-weight urinary proteins, rather than increased synthesis of these proteins, appears to cause hyaline droplet accumulation.