Cell- and noncell-autonomous AUXIN RESPONSE FACTOR3 controls meristem proliferation and phyllotactic patterns
Cell- and noncell-autonomous AUXIN RESPONSE FACTOR3 controls meristem proliferation and phyllotactic patterns
复制标题
细胞和非细胞自主生长素反应因子3控制分生组织增殖和叶序模式
DOI：
10.1093/plphys/kiac370
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发表时间：
2022-08-16
期刊：
影响因子：
7.4
通讯作者：
Liu, Xigang
中科院分区：
文献类型：
--
作者：
Zhang, Ke;Zhang, Hao;Liu, Xigang
在细胞间的通讯中，非细胞自主转录因子在控制植物干细胞命运中起着至关重要的作用。生长素反应因子3(ARF3)是ARF家族中的一个成员，在花分生组织的维持和决定中起关键作用，它具有不同于其编码基因在顶端分生组织(SAM)中的表达结构域的独特积累模式。然而，这种差异的生物学意义并不明确。在这里，我们证明了ARF3在拟南芥(Arabiopsis Thaliana)中的表达主要由生长素激活，其中ARF3细胞自主调节分生组织-器官边界特异基因的表达，如杯状COTYLEDON1-3(CUC1-3)、PETIOLE1-2上的叶片(BOP1-2)和ETTIN CONTROL3(TEC3)下的靶标(TEC3)以调节器官的规则排列，这种现象被称为叶序。我们还发现ARF3被移位到组织中心，在那里它抑制细胞分裂素活性和WUSCHEL表达，以非细胞自主的方式调节分生组织的活动。因此，ARF3在调节分生组织维持和器官发生之间的平衡的同时，在SAM中起着调节生长素和细胞分裂素信号相互作用的分子链接的作用。我们的发现揭示了ARF3在动态SAM维持中通过细胞-细胞通讯介导的协调机制。AUXIN反应因子3抑制细胞分裂素活性和WUSCHEL表达，以非细胞自主的方式协调分生组织的活动，同时它自主地调节叶状模式细胞。
In cell-cell communication, noncell-autonomous transcription factors play vital roles in controlling plant stem cell fate. We previously reported that AUXIN RESPONSE FACTOR3 (ARF3), a member of the ARF family with critical roles in floral meristem maintenance and determinacy, has a distinct accumulation pattern that differs from the expression domain of its encoding gene in the shoot apical meristem (SAM). However, the biological meaning of this difference is obscure. Here, we demonstrate that ARF3 expression in Arabidopsis (Arabidopsis thaliana) is mainly activated at the periphery of the SAM by auxin where ARF3 cell autonomously regulates the expression of meristem-organ boundary-specific genes, such as CUP-SHAPED COTYLEDON1-3 (CUC1-3), BLADE ON PETIOLE1-2 (BOP1-2), and TARGETS UNDER ETTIN CONTROL3 (TEC3) to regulate the arrangement of organs in regular pattern, a phenomenon referred to as phyllotaxis. We also show that ARF3 is translocated into the organizing center where it represses cytokinin activity and WUSCHEL expression to regulate meristem activity noncell-autonomously. Therefore, ARF3 acts as a molecular link that mediates the interaction of auxin and cytokinin signaling in the SAM while coordinating the balance between meristem maintenance and organogenesis. Our findings reveal an ARF3-mediated coordination mechanism through cell-cell communication in dynamic SAM maintenance.AUXIN RESPONSE FACTOR3 represses cytokinin activity and WUSCHEL expression to coordinate meristem activity noncell-autonomously, while it regulates phyllotactic patterning cell-autonomously.