Characterization of pollen cube development in Pinus strobus (Eastern white pine) through proteomic analysis of differentially expressed proteins
Characterization of pollen cube development in Pinus strobus (Eastern white pine) through proteomic analysis of differentially expressed proteins
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DOI：
10.1002/pmic.200500009
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发表时间：
2005-12-01
期刊：
影响因子：
3.4
通讯作者：
Fernando, DD
中科院分区：
文献类型：
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作者：
Fernando, DD
这些差异表达的蛋白质表明了它们在雄配子体发育过程中的特殊作用。为了分离这些蛋白质，用未萌发的花粉和2日龄的花粉管进行了2-DE。结果表明，在花粉粒和花粉管中分别分离出645和647个蛋白质点。38个蛋白质点仅在花粉管中表达，而19个蛋白质点的强度增加。使用MALDI-TOF MS生成胰蛋白酶肽质量，提交给Mascot进行鉴定。在差异表达的蛋白质中，12%与假设的蛋白质匹配，33%没有命中任何蛋白质，并且对于55%，基于与先前表征的蛋白质的序列相似性分配推定的功能。因此，花粉管发育可以通过涉及代谢、胁迫/防御反应、基因调控、信号转导和细胞壁形成的细胞活动来表征。这项研究扩展了我们对与花粉管发育相关的蛋白质表达变化的理解，并提供了将花粉管发育与花粉粒分离的分子程序的见解。这是第一份报告，描述了一个全球性的分析差异表达的蛋白质从任何种子植物的花粉管。
The differentially expressed proteins in pollen tubes indicate their specific roles in this stage of male gametophyte development. To isolate these proteins, 2-DE was done using ungerminated pollen and 2-day-old pollen tubes of Pinus strobus. Results show that 645 and 647 protein spots were clearly resolved from pollen grains and pollen tubes, respectively. Thirty-eight protein spots were expressed only in pollen tubes, while 19 increased in intensity. MALDI-TOF MS was used to generate tryptic peptide masses that were submitted to Mascot for identification. Of the differentially expressed proteins, 12% matched with hypothetical proteins, 33% did not hit any protein, and for the 55%, a putative function was assigned based on similarity of sequences with previously characterized proteins. Therefore, pollen tube development can be characterized by the cellular activities that involve metabolism, stress/defense response, gene regulation, signal transduction, and cell wall formation. This study expands our understanding of the changes in protein expression associated with pollen tube development and provides insights into the molecular programs that separate the development of the pollen tubes from pollen grains. This is the first report that describes a global analysis of differentially expressed proteins from the pollen tube of any seed plant.