Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with preharvest sprouting tolerance
Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with preharvest sprouting tolerance
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DOI：
10.1007/s001220051241
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发表时间：
1999-07-01
影响因子：
5.4
通讯作者：
Gupta, PK
中科院分区：
文献类型：
--
作者：
Roy, JK;Prasad, M;Gupta, PK
在面包小麦中，使用常规植物育种方法难以将与具有红色籽粒的基因型相关的收获前发芽（PI-IS）耐受性转移到具有琥珀色籽粒的基因型。本研究旨在鉴定与PI-IS耐受性相关的DNA标记，因为这些标记将允许间接标记辅助选择具有琥珀色籽粒的PI-IS耐受基因型。为此目的，使用具有红色籽粒的PI-IS耐受基因型SPR 8198和具有白色籽粒的PI-IS敏感品种“HD 2329”之间的杂交培育了一组100个重组自交系（RILs）。用232个序列标记微卫星位点（STMS）和138个序列标记位点（STS）引物对两个亲本进行了分析。通过给出可评分的PCR产物，总共300个（167个STMS和133个STS）引物对证明了功能。其中，57（34%）STMS和30（23%）STS引物对检测到双亲基因型之间的重复多态性。使用这些引物对，我们进行了两个散装的DNA，一个通过汇集DNA从5个PHS-耐受RILs和其他类似的来自汇集DNA从5个PHS-敏感RILs的散装分离分析。两个分子标记，一个用于基因座wmc 104的STMS引物对和一个用于基因座MST 101的STS引物对。显示与PI-IS耐受性有明显联系。在对原液中包含的单个RILs进行选择性基因分型后，证实了这一点。对100个RILs的共分离数据进行了独立性卡方列联检验，这些RILs涉及两个分子标记（wmc 104和MST 101）和PHS。这些测试揭示了每个标记与对PHS的耐受性之间的强关联。使用缺体-四体系，我们能够将wmc 104和MST 101分别分配到染色体GB和7 D。这一结果还表明，SPR 8198对Pi-IS的耐受性可能是由两个基因（与两个分子标记连锁）互补互作决定的。
In bread wheat, the transfer of tolerance to preharvest sprouting (PI-IS) that is associated with genotypes having red kernel colour to genotypes with amber kernels is difficult using conventional methods of plant breeding. The study here was undertaken to identify DNA markers linked with tolerance to PI-IS as these would allow indirect marker-assisted selection of PI-IS-tolerant genotypes with amber kernels. For this purpose, a set of 100 recombinant inbred lines (RILs) was developed using a cross between a PI-IS-tolerant genotype, SPR8198, with red kernels and a PI-IS-susceptible cultivar,'HD2329', with white kernels. The two parents were analysed with 232 STMS (sequence-tagged microsatellite site) and 138 STS (sequence-tagged site) primer pairs. A total of 300 (167 STMSs and 133 STSs) primer pairs proved functional by giving scorable PCR products. Of these, 57 (34%) STMS and 30 (23%) STS primer pairs detected reproducible polymorphism between the parent genotypes. Using these primer pairs, we carried out bulked segregant analysis on two bulked DNAs, one obtained by pooling DNA from 5 PHS-tolerant RILs and the other similarly derived by pooling DNA from 5 PHS-susceptible RILs. Two molecular markers, I STMS primer pair for the locus wmc104 and a STS primer pair for the locus MST101. showed apparent linkage with tolerance to PI-IS. This was confirmed following selective genotyping of individual RILs included in the bulks. Chi-square contingency tests for independence were conducted on the cosegregation data collected on 100 RILs involving each of the two molecular markers (wmc104 and MST101) and PHS. The tests revealed a strong association between each of the markers and tolerance to PHS, Using nullisomic-tetrasomic lines, we were able to assign wmc104 and MST101 to chromosomes GB and 7D, respectively. The results also indicated that the tolerance to Pi-IS in SPR8198 is perhaps governed by two genes (linked with two molecular markers) exhibiting complementary interaction.