Studies on the Antibacterial Activity and Mechanism of Antimicrobial Peptides Against Drug-Resistant Bacteria
Studies on the Antibacterial Activity and Mechanism of Antimicrobial Peptides Against Drug-Resistant Bacteria
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DOI：
10.1166/jbn.2018.2528
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发表时间：
2018-03-01
影响因子：
2.9
通讯作者：
Ouyang, Xiaoping
中科院分区：
文献类型：
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作者：
Luo, Xiaofang;Liu, Yanjun;Ouyang, Xiaoping
抗生素耐药性对人类健康的威胁越来越大。最重要的是，多重耐药革兰氏阴性菌对医疗保健构成了最大的挑战，这主要是由于缺乏有效的治疗选择。在本研究中，我们研究了肽HX-12 A、HX-12 B和HX-12 C对MDRAB和MDRKP分离株的体外活性。这三种肽对MDRAB和MDRKP分离株均表现出较高的抗菌活性和快速杀菌作用。此外，即使存在于pH 6.8和8.0、盐（NaCl 100 mM、CaCl 2 1 mM、MgCl 2 1 mM）或人血清（5%）中，肽仍保持其活性，尤其是肽HX-12 C。此外，杀菌动力学和透射电子显微镜结果表明，肽的杀菌机制可能是破坏细胞膜，这使得细菌更难产生耐药性。因此，这三种肽有可能被开发成新的抗菌剂。
Antibiotic resistance is a growing threat to human health. More than anything else, multidrug-resistant gram-negative bacteria pose the biggest challenge to healthcare, which is predominantly due to the lack of effective therapeutic options. In the present study, we investigated the activities of peptides HX-12A, HX-12B and HX-12C against MDRAB and MDRKP isolates in vitro. Those three peptides displayed high antibacterial activities and rapid bactericidal effects against MDRAB and MDRKP isolates. Additionally, the peptides retained their activity even being present in pH 6.8 and 8.0, salt (NaCl 100 mM, CaCl2 1 mM, MgCl2 1 mM) or human serum (5%), especially peptide HX-12C. Moreover, the killing kinetics and transmission electron microscopy results suggested that the possible sterilization mechanism of peptides is to destroy the cell membrane, which makes it more difficult for bacteria to develop resistance. Thus, these three peptides may be developed into new antimicrobial agents.