Cloning and sequence determination of bovine insulin‐like growth factor binding protein‐2 (IGFBP‐2): Comparison of its structral and functional properties with IGFBP‐1
Cloning and sequence determination of bovine insulin‐like growth factor binding protein‐2 (IGFBP‐2): Comparison of its structral and functional properties with IGFBP‐1
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牛胰岛素样生长因子结合蛋白-2（IGFBP-2）的克隆和序列测定：其与IGFBP-1的结构和功能特性的比较
DOI：
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发表时间：
1992
影响因子：
4
通讯作者：
G. Krivi
中科院分区：
文献类型：
--
作者：
W. Busby;R. McCusker;D. Clemmons;M. Bourner;N. Siegel;G. Krivi
胰岛素样生长因子结合蛋白(IGFBPs)由多种类型的细胞分泌，可以改变IGF的作用。Mandin-Darby牛肾(MDBK)细胞分泌34000Da的IGF结合蛋白，其N端序列与从大鼠BRL-3A细胞中纯化的IGFBP-2相似。这些研究报道了牛IGFBP-2的完整氨基酸序列，并与人IGFBP-1进行了比较。该蛋白与大鼠IGFBP-2有81%的同源性。与大鼠IGFBP-2和人IGFBP-1相比，所有18个半胱氨酸残基的位置都是保守的。类似地，在两种蛋白质的羧基末端附近都存在RGD序列。IGFBP-2与IGF-II的亲和力高于与IGF-I的亲和力，与人IGFBP-1的亲和力大于与IGF-II的亲和力。与IGFBP-1一样，IGFBP-2蛋白能增强猪主动脉平滑肌细胞对IGF-I的DNA合成反应，但IGFBP-2的亲和力要弱得多。IGF介导的有丝分裂反应的最大增强作用约为IGFBP-1的42%。这种增强依赖于血小板贫乏血浆中包含的一种因子，如果从孵育液中省略这一因子，IGFBP-2就会抑制DNA合成。IGFBP-2的提纯将允许在生理测试系统中将其与其他形式的IGFBP进行更详细的比较。
Insulin‐like growth factor binding proteins (IGFBPs) are secreted by several cell types and can modify IGF actions. Mandin‐Darby Bovine Kidney (MDBK) cells have been shown to secrete a 34,000 Da form of IGF binding protein whose N‐terminal sequence is similar to a form of IGFBP purified from rat BRL‐3A cells that has recently been named IGFBP‐2. These studies report the complete amino acid sequence of bovine IGFBP‐2 and compare its functional properties with human IGFBP‐1. The protein is 81% identical to rat IGFBP‐2. When compared with both rat IGFBP‐2 and human IGFBP‐1, the positions of all 18 cysteine residues are conserved. Similarly an RGD sequence is present near the carboxyl terminus in both proteins. IGFBP‐2 has a higher affinity for IGF‐II than for IGF‐I and its affinity for both forms of IGF is greater than for human IGFBP‐1. Like IGFBP‐1 the protein can enhance the DNA synthesis response of porcine aortic smooth muscle cells to IGF‐I; however, IGFBP‐2 was much less potent. The maximum potentiation of the IGF‐mediated mitogenic response that could be achieved was approximately 42% that of IGFBP‐1. This potentiation is dependent upon a factor contained in platelet poor plasma and if this factor is omitted from the incubation medium, IGFBP‐2 inhibits DNA synthesis. The purification of IGFBP‐2 will allow more detailed comparisons to be made between it and other forms of IGFBPs in physiologic test systems.
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DOI：
10.1073/pnas.88.17.7481
发表时间：
1991-09-01
影响因子：
11.1
作者：
JONES, JI;DERCOLE, AJ;CLEMMONS, DR
通讯作者：
CLEMMONS, DR
羊水来源的胰岛素样生长因子结合蛋白的纯化制剂含有具有生物活性的多聚体形式。
影响因子：
4.8
作者：
Busby,WH;Hossenlopp,P;Binoux,M;Clemmons,DR
通讯作者：
Clemmons,DR
胰岛素样生长因子 I 分子的离散改变会改变其与胰岛素样生长因子结合蛋白的亲和力，从而导致生物活性的变化。
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Clemmons,DR;Cascieri,MA;Camacho-Hubner,C;McCusker,RH;Bayne,ML
通讯作者：
Bayne,ML
成纤维细胞表面相关 35 K 蛋白合成的改变可调节生长调节素-C/胰岛素样生长因子 I 的结合。
DOI：
10.1210/mend-1-5-339
发表时间：
1987
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Clemmons,DR;Han,VK;Elgin,RG;D'Ercole,AJ
通讯作者：
D'Ercole,AJ
将胰岛素样生长因子和结合蛋白活性释放到培养的人成纤维细胞的无血清培养基中。
影响因子：
4.8
作者：
Adams,SO;Kapadia,M;Mills,B;Daughaday,WH
通讯作者：
Daughaday,WH