Laser-Induced Forward Transfer: A Laser-Based Technique for Biomolecules Printing
Laser-Induced Forward Transfer: A Laser-Based Technique for Biomolecules Printing
复制标题
激光诱导正向转移：基于激光的生物分子打印技术
DOI：
10.1007/978-90-481-9145-1_4
复制
发表时间：
2010
影响因子：
19
通讯作者：
J. Morenza
中科院分区：
文献类型：
--
作者：
P. Serra;M. Duocastella;J. Fernández;J. Morenza
激光的高聚焦功率使其适用于微图案化应用。激光诱导前向转移(LIFT)是一种直接写入技术，它允许通过脉冲激光的作用将微量的材料从施主薄膜沉积到固体衬底上。虽然Lift最初被开发为与固体薄膜一起运行，但已经证明，从液体薄膜也可以沉积。在这种情况下，材料直接以液体状态从薄膜中喷射出来，并转移到受体衬底，在那里以微滴的形式沉积。薄膜-衬底系统相对于激光的相对平移使得能够形成二维图案。这使得Lift足够用于生物分子打印：生物溶液的微滴可以转移到固体基质上，以产生固定的生物分子图案。本章从生物分子印刷的起源到最新进展，对生物分子印刷的升降技术进行了综述。详细描述了该技术的特点和性能，并特别注意了各种可能的操作模式和传递机制。它还表明，与其他更传统的直接写入方法相比，通过Lift可以在分辨率、速度、污染和样品消耗方面获得显著的好处。最后，通过成功沉积大量不同生物分子的实例，论证了该技术用于生物分子印刷的可行性。
The high focusing power of lasers makes them adequate for micropatterning applications. Laser-induced forward transfer (LIFT) is a direct-writing technique allowing the deposition of tiny amounts of material from a donor thin film to a solid substrate through the action of a pulsed laser beam. Although LIFT was originally developed to operate with solid films, it has been demonstrated that deposition is also possible from liquid films. In this case the material is directly ejected in the liquid state from the film and transferred to the receptor substrate, where it deposits in the form of a microdroplet. The relative translation of the film-substrate system respect to the laser beam enables the formation of two-dimensional patterns. This makes LIFT adequate for biomolecule printing: microdroplets of biological solutions can be transferred onto solid substrates to produce patterns of immobilized biomolecules. In this chapter a review on the LIFT technique for biomolecule printing is carried out, from its origins to the most recent developments. The characteristics and performances of the technique are described in detail, with special attention to the diverse possible modes of operation and transfer mechanisms. It is also shown that significant benefits in terms of resolution, speed, contamination, and sample consumption can be obtained through LIFT when compared to other more conventional direct-writing approaches. Finally, the feasibility of the technique for biomolecule printing is demonstrated through examples of successful deposition of a large set of different biomolecules.