Direct molecular Imaging of Lymnaea stagnalis nervous tissue at subcellular spatial resolution by mass spectrometry
Direct molecular Imaging of Lymnaea stagnalis nervous tissue at subcellular spatial resolution by mass spectrometry
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DOI：
10.1021/ac048329g
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发表时间：
2005-02-01
影响因子：
7.4
通讯作者：
Piersma, SR
中科院分区：
文献类型：
--
作者：
Altelaar, AFM;van Minnen, J;Piersma, SR
结合飞行时间二次离子质谱（ToF-SIMS）和MALDI-MS样品制备方法的成像能力。我们使用这种称为矩阵增强（ME）西姆斯的方法以微米空间分辨率对神经组织进行直接分子成像。将淡水蜗牛的大脑神经节的冷冻切片置于铟锡氧化物（ITO）涂层的导电玻璃载玻片上，并通过电喷雾沉积用2，5-二羟基苯甲酸薄层覆盖。构建了胆固醇和神经肽APGW酰胺的高分辨率分子离子图。APGWamide主要定位于调节雄性交配行为的神经元簇中。ME-SIMS成像允许直接从组织切片进行分子特异性成像而无需标记，并打开互补的质量窗口（
The imaging capabilities of time-of-flight secondary ion mass spectrometry (ToF-SIMS) and MALDI-MS sample preparation methods were combined. We used this method, named matrix-enhanced (ME) SIMS, for direct molecular imaging of nervous tissue at micrometer spatial resolution. Cryosections of the cerebral ganglia of the freshwater snail Lymnaea stagnalis were placed on indium-tin-oxide (ITO)-coated conductive glass slides and covered with a thin layer of 2,5-dihydroxybenzoic acid by electrospray deposition. High-resolution molecular ion maps of cholesterol and the neuropeptide APGWamide were constructed. APGWamide was predominantly localized in the cluster of neurons that regulate male copulation behavior of Lymnaea. ME-SIMS imaging allows direct molecule-specific imaging from tissue sections without labeling and opens a complementary mass window (