Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori
Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori
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DOI：
10.1007/s10529-008-9898-8
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发表时间：
2009-04-01
影响因子：
2.7
通讯作者：
Kusakabe, Takahiro
中科院分区：
文献类型：
--
作者：
Karasaki, Noriko;Mon, Hiroaki;Kusakabe, Takahiro
四环素诱导的基因表达系统（Tet-on）已成为控制哺乳动物和果蝇转基因表达的首选系统之一。然而，已经在哺乳动物系统中建立的Tet-on系统或在果蝇中调谐的Tet-on系统在家蚕中不起作用。在B中构建功能性Tet-on系统。在rtTA中引入了苜蓿银纹夜蛾核型多角体病毒（Autographa californica nuclear polyhedrosis virus，简称ALPV）即早基因1的转录激活结构域和SV 40大T抗原的核定位信号。在多西环素存在下，修饰的rtTA可以激活家蚕细胞中从9 × tetO启动子的转录高达250倍。
Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 x tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.