Osteoprogenitor cells within skeletal muscle
Osteoprogenitor cells within skeletal muscle
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DOI：
10.1002/jor.1100180613
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发表时间：
2000-11-01
影响因子：
2.8
通讯作者：
Huard, J
中科院分区：
文献类型：
--
作者：
Bosch, P;Musgrave, DS;Huard, J
骨骼肌内异位骨的形成是一个被广泛观察到的现象。然而，负责异位骨形成的骨祖细胞的来源仍不清楚。这项研究旨在测试从骨骼肌组织分离的细胞之间的成骨分化。不同亚群的成年小鼠骨骼肌细胞在体外暴露于骨形态发生蛋白-2后，检测碱性磷酸酶的活性。用编码β-半乳糖苷酶的逆转录病毒(RV-LacZ)和编码一种骨形态发生蛋白-2的腺病毒载体转导一个有反应的亚群，并注射到免疫受损(严重联合免疫缺陷，SCID)小鼠的后肢。注射的细胞似乎积极参与了异位骨的形成。在新形成的骨基质陷窝中，LacZ阳性的肌源性细胞与骨钙素产生细胞共存，提示成骨细胞和骨细胞分化。尽管没有分离出特定的细胞，但这些数据支持骨祖细胞存在于骨骼肌内，肌肉可能是获取这些细胞的骨髓以外的来源的观点。
The formation of ectopic bone within skeletal muscle is a widely observed phenomenon. However, the source of the osteoprogenitor cells responsible for ectopic bone formation remains unknown. This study was designed to test for osteogenic differentiation among cells isolated from skeletal muscle tissue. Different subpopulations of cells derived from an adult mouse skeletal muscle were tested for induction of alkaline phosphatase activity after exposure to bone morphogenetic protein-2 in vitro. A responsive subpopulation was identified, transduced with a retrovirus encoding for beta -galactosidase (Rv-lacZ) and an adenoviral construct encoding for one bone morphogenetic protein-2, and injected into the hindlimb of immune compromised (severe combined immunodeficient, or SCID) mice. The injected cells appeared to actively participate in the ectopic bone formation. The existence of lacZ-positive muscle-derived cells colocalized with osteocalcin-producing cells within lacunae of newly formed bone matrix suggests osteoblast and osteocyte differentiation. Although a specific cell was not isolated, these data support the contentions that osteoprogenitor cells reside within skeletal muscle and that muscle may represent a source other than bone marrow for the harvest of these cells.