THE BEHAVIOR OF SONICATED ALBUMIN MICROBUBBLES WITHIN THE MICROCIRCULATION - A BASIS FOR THEIR USE DURING MYOCARDIAL CONTRAST ECHOCARDIOGRAPHY
THE BEHAVIOR OF SONICATED ALBUMIN MICROBUBBLES WITHIN THE MICROCIRCULATION - A BASIS FOR THEIR USE DURING MYOCARDIAL CONTRAST ECHOCARDIOGRAPHY
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DOI：
10.1161/01.res.65.2.458
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发表时间：
1989-08-01
影响因子：
20.1
通讯作者：
DULING, B
中科院分区：
文献类型：
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作者：
KELLER, MW;SEGAL, SS;DULING, B
这项研究的目的是确定超声白蛋白微泡的行为是否准确地模拟了微循环中的红细胞流动，从而与它们在心肌声学造影期间用作红细胞流动的活体示踪剂的使用一致。相应地，将荧光素偶联白蛋白和荧光标记的红细胞制成的微泡注入8只金黄地鼠的血管内。在颊囊微循环中测定其血管内分布、血流速度、小动脉到小静脉的通过时间和分支处的流量比率。白蛋白微泡(平均直径4.9.+-.3.6µm)和红细胞在小动脉管腔内的分布频率相似(33%在小动脉中央20%)，它们的小动脉血流速度也相似(2.5.+-)。0.7毫米/秒和2.3.+-0.7 mm/s，p=NS)。微泡的平均速度与应用腺苷后红细胞的平均速度有很好的相关性(r=0.97和r=0.89)，计算的最大速度也是如此(基线和腺苷分别为r=0.98和0.80)。白蛋白微泡和红细胞的管腔速度曲线在基线和腺苷诱导的速度变化后相似。各分支点的通量比也具有良好的相关性(r=0.92p<；0.001)。白蛋白微泡从微动脉到静脉的转运时间与大小从22微米到45微米的血管中的红细胞相似。我们得出结论，白蛋白微泡在微循环中的行为类似于红细胞的行为，并支持在心肌声学造影中将其用作红细胞流动的血管内示踪剂。
The purpose of this study was to determine whether the behavior of sonicated albumin microbubbles accurately mimics red blood cell flow in the microcirculation and is thus consistent with their use as in vivo tracers of red blood cell flow during myocardial contrast echocardiography. Accordingly, microbubbles prepared from fluorescein-conjugated albumin and fluorescently labeled red blood cells were injected intravascularly in eight golden hamsters. Their intravascular distribution, velocities, arteriolar-to-venular transit times, and flux ratios at branch points were determined in the microcirculation of the cheek pouch. Albumin microbubbles (mean diameter, 4.9 .+-. 3.6 .mu.m) and red blood cells displayed a similar frequency of distribution across the arteriolar lumen (33% in the central 20% of the arterioles), and their arteriolar velocities were also similar (2.5 .+-. 0.7 mm/sec and 2.3 .+-. 0.7 mm/sec, p = NS). The mean velocities of microbubbles correlated well with those of red blood cells at baseline and after adenosine application (r = 0.97 and r = 0.89, respectively), as did the calculated maximum velocity (r = 0.98 and r = 0.80, baseline and adenosine, respectively). The velocity profiles across the lumen of the vessels for albumin microbubbles and red blood cells were similar at baseline and after adenosine-induced velocity changes. The flux ratios at branch points also correlated well (r = 0.92, p < 0.001). Arteriolar-to-venular transit times of albumin microbubbles were similar to those of red blood cells in vessels ranging in size from 22 .mu.m to 45 .mu.m. We conclude that the behavior of albumin microbubbles in the microcirculation mimics that of red blood cells and supports their use as intravascular tracers of red blood cell flow during myocardial contrast echocardiography.