Comparison of Rapid DNA Extraction Methods Applied to PCR Identification of Medicinal Mushroom Ganoderma spp.
Comparison of Rapid DNA Extraction Methods Applied to PCR Identification of Medicinal Mushroom Ganoderma spp.
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DOI：
10.1080/10826060701593282
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发表时间：
2007-09
影响因子：
2.9
通讯作者：
Xuanwei Zhou;Qi-Zhang Li;Jing-ya Zhao;K. Tang;Juan Lin;Yizhou Yin
中科院分区：
文献类型：
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作者：
Xuanwei Zhou;Qi-Zhang Li;Jing-ya Zhao;K. Tang;Juan Lin;Yizhou Yin
摘要采用4种不同的DNA提取方法，从灵芝菌丝体、干燥子实体、孢子粉切片和破壁孢子粉中提取灵芝基因组DNA。采用琼脂糖凝胶电泳、紫外分光光度计和PCR扩增对DNA样品进行分析。从DNA的平均得率和平均纯度来看，高盐低pH法是最佳的DNA提取方法。菌丝体和孢子壁破碎的孢子粉产生更高和更纯的DNA。该方法能有效消除次生代谢产物对DNA提取的影响。从开发的方法提取的DNA样品可以成功地用于PCR应用。
Abstract Four different DNA extraction methods were used to extract genomic DNA of the medicinal mushroom Lingzhi from its developing stage materials, such as mycelium, dry fruiting body, or sliced and spore powder or sporoderm‐broken spore powder. The DNA samples were analyzed using agarose gel electrophoresis, UV spectrophotometer, and PCR amplification. According to the average yields and purity of DNA, high salt concentrations and low pH methods were the best for DNA extraction. The mycelia and sporoderm‐broken spore powder yielded higher and purer DNA. The method developed could effectively eliminate the influence of the secondary metabolites to DNA extraction. The DNA samples extracted from the developed method could be successfully used for PCR applications.