Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae.
Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae.
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STE6 基因上游的序列，是酿酒酵母交配型基因座表达和调节所需的。
DOI：
10.1073/pnas.83.8.2536
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发表时间：
1986
影响因子：
11.1
通讯作者：
Herskowitz,I
中科院分区：
文献类型：
--
作者：
Wilson,KL;Herskowitz,I
酿酒酵母的STE 6基因是α-特异性基因;它在α细胞中被交配型基因座的α 2产物抑制。为了研究STE 6上游序列在其调控和表达中的作用，我们确定了启动子区的DNA序列，确定了STE 6转录的起始位点，并确定了控制其转录的序列。产生去除STE 6基因上游DNA的缺失，并测定其对调控和表达的影响。缺失定义了STE 6转录起始位点上游的两个间隔。一个包含全部或部分消极因素;另一个包含全部或部分积极因素。负元件是通过α 2抑制STE 6所必需的：缺失该元件组成性表达STE 6。这种缺失去除了位于第一转录起始位点上游135个碱基对的31个碱基对位点，该位点与存在于被α 2抑制的其他四个基因的上游区域中的位点高度同源。这些位点可能是α 2对α-特异性基因的抑制的原因。阳性元件（推定的上游活化位点）是STE 6表达所必需的。缺失定义了拟定上游激活位点的左侧边界。STE 6和其他α-特异性基因之间的序列同源性被发现在该区域中，并可能介导这组基因的激活。
The STE6 gene of Saccharomyces cerevisiae is an a-specific gene; it is repressed in alpha cells by the alpha 2 product of the mating type locus. To study the role of sequences upstream of STE6 in its regulation and expression, we have determined the DNA sequence of the promoter region, identified the start sites for the STE6 transcript, and identified sequences governing its transcription. Deletions that remove DNA upstream of the STE6 gene were produced and assayed for effects on regulation and expression. The deletions defined two intervals upstream of the STE6 transcription initiation sites. One contains all or part of a negative element; the other contains all or part of a positive element. The negative element is required for repression of STE6 by alpha 2: deletions lacking this element express STE6 constitutively. Such deletions remove a 31-base-pair site, located 135 base pairs upstream of the first transcript start site, that is highly homologous to sites present in the upstream regions of four other genes repressed by alpha 2. These sites are presumably responsible for repression of the a-specific genes by alpha 2. The positive element (a putative upstream activation site) is required for expression of STE6. The deletions define the left boundary of the proposed upstream activation site. Sequence homologies between STE6 and other a-specific genes are found in this region and may mediate activation of this set of genes.