Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples.
Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples.
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DOI：
10.1117/1.3523368
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发表时间：
2010-11
影响因子：
3.5
通讯作者：
Chun Wei Wang;U. Manne;V. Reddy;D. Oelschlager;V. Katkoori;W. Grizzle;R. Kapoor
中科院分区：
文献类型：
--
作者：
Chun Wei Wang;U. Manne;V. Reddy;D. Oelschlager;V. Katkoori;W. Grizzle;R. Kapoor
已经开发出一种组合锥形光纤生物传感器 (CTFOB) 浸入式探针，用于快速且经济高效地定量血清样品中的蛋白质。该装置依赖于二极管激光激发和电荷耦合装置光谱仪，并基于夹心免疫分析技术发挥作用。作为原理证明，该技术被应用于白细胞介素 IL-6 的定量估计。这些探针在取自狼疮、自身免疫性疾病和淋巴瘤患者的血清样本中检测到皮摩尔水平的 IL-6。狼疮样本中 IL-6 的估计浓度为 5.9 ± 0.6 pM，而淋巴瘤样本中的 IL-6 浓度低于检测限。这些浓度通过涉及基于微珠的 xMAP 技术的程序进行验证。观察到类似的浓度趋势。通过分析接收器操作特性来评估 CTFOB 浸入式探头的特异性。该分析表明浸入式探针可以检测这些样品中 5 pM 或更高浓度的 IL-6，特异性为 100%。这些结果为指导进一步研究利用这些探针以高特异性和灵敏度量化体液中的其他分析物提供了信息。
A combination tapered fiber-optic biosensor (CTFOB) dip probe for rapid and cost-effective quantification of proteins in serum samples has been developed. This device relies on diode laser excitation and a charged-coupled device spectrometer and functions on a technique of sandwich immunoassay. As a proof of principle, this technique was applied in a quantitative estimation of interleukin IL-6. The probes detected IL-6 at picomolar levels in serum samples obtained from a patient with lupus, an autoimmune disease, and a patient with lymphoma. The estimated concentration of IL-6 in the lupus sample was 5.9 ± 0.6 pM, and in the lymphoma sample, it was below the detection limit. These concentrations were verified by a procedure involving bead-based xMAP technology. A similar trend in the concentrations was observed. The specificity of the CTFOB dip probes was assessed by analysis with receiver operating characteristics. This analysis suggests that the dip probes can detect 5-pM or higher concentration of IL-6 in these samples with specificities of 100%. The results provide information for guiding further studies in the utilization of these probes to quantify other analytes in body fluids with high specificity and sensitivity.