Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei.
Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei.
复制标题
四膜虫接合诱导基因：大核和微核的结构和组织。
DOI：
10.1093/nar/14.3.1341
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发表时间：
1986
影响因子：
14.9
通讯作者：
Bruns,PJ
中科院分区：
文献类型：
--
作者：
Martindale,DW;Martindale,HM;Bruns,PJ
在该生物体的体细胞核（宏观）和生发细胞核（微观）中检查了在接合（减数分裂）期间活跃的八个四膜膜基因的物理组织。其中三个基因产生的转录物仅在减数分裂前期检测到。 Southern印迹分析表明所有检查的基因都存在于两个细胞核中。除了一个基因家族（pC6）外，所有基因家族似乎都是非重复的，并且在基因内部或附近没有可检测到的序列重排。这个特殊的基因被重复了大约五到七次，DNA重排发生在每个拷贝中或附近。 cDNA 与大核和微核 DNA 限制性图谱的比较表明，其中一个基因 (cnj B) 含有内含子。这是四膜虫非核糖体基因内含子证据的第一份报告。在另一个基因 (cnj C) 的大核拷贝中发现了可能由腺嘌呤甲基化引起的位点特异性修饰。
The physical organization of eightTetrahymenagenes active during conjugation (meiosis) was examined in the somatic (macro-) and germinal (micro-) nuclei of this organism. Three of these genes make transcripts that are only detected during meiotic prophase. Southern blot analyses indicated that all genes examined were present in both nuclei. Except for one gene family (pC6), all appeared to be non-repetitive and there were no detectable sequence rearrangements in or near the genes. The exceptional gene was repeated approximately five to seven times and DNA rearrangement occurred in or near each of these copies. A comparison of cDNA and macro- and micronuclear DNA restrication maps indicated that one of the genes (cnj B) contains introns. This is the first report of evidence for introns in a non-ribosomal gene inTetrahymena. A site specific modification probably due to adenine methylation was seen in the macronuclear copy of another gene (cnj C).