Fabrication of a Corneal Epithelial Cell Sheet from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell Differentiation
Fabrication of a Corneal Epithelial Cell Sheet from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell Differentiation
复制标题
基于眼细胞自发分化的方法从人多能干细胞制备角膜上皮细胞片
DOI：
10.1038/protex.2016.009
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Hayashi R
中科院分区：
文献类型：
--
作者：
Hayashi R
我们已经建立了一种新的培养系统，通过从人诱导多能干细胞（hiPSC）自发发育眼细胞来诱导角膜上皮的形成。hiPSC衍生的角膜上皮细胞（hiCEC）可以通过FACS用特异性抗体纯化，并且具有重建表达p63、K12和PAX6的功能性角膜上皮的能力。
We have established a novel culture system to induce the formation of corneal epithelium through the spontaneous development of ocular cells from human induced pluripotent stem cells (hiPSCs). The hiPSC derived-corneal epithelial cells (hiCECs) can be purified by FACS with specific antibodies and have the ability to reconstruct functional corneal epithelium expressing p63, K12, and PAX6.