Alu insertion loci and platyrrhine primate phylogeny
Alu insertion loci and platyrrhine primate phylogeny
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DOI：
10.1016/j.ympev.2004.10.023
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发表时间：
2005-04-01
影响因子：
4.1
通讯作者：
Batzer, MA
中科院分区：
文献类型：
--
作者：
Ray, DA;Xing, J;Batzer, MA
短插入元件（西内斯）是非常有用的系统发育标记，因为特定元件在基因组中某一位置的整合是不可逆的，并且具有已知的极性。这些属性使分析西内斯作为系统发育特征基本上是无同质性的事情。Alu元件是灵长类特异性的西内斯，其构成人类基因组的很大一部分，并且在其他灵长类动物中也广泛存在。使用组合湿台和计算方法，我们恢复了190个Alu插入，其中183个是特定于9个新世界灵长类动物的基因组。我们用这些位点来调查分支顺序，并产生了支持Atelidae（蜘蛛，羊毛，吼猴）和Cebidae（绒猴，绢毛猴，猫头鹰猴）之间的姐妹关系，然后加入这两个家庭的分支Pitheciidae（titi和萨基猴）。数据支持这些关系的同质性指数为0.00。在这项研究中，我们报告了一个最大的应用SINE元素的系统发育分析，到目前为止，和结果提供了一个强大的分子系统发育的阔鼻灵长类动物。(c)2004爱思唯尔公司All rights reserved.
Short INterspersed Elements (SINEs) make very useful phylogenetic markers because the integration of a particular element at a location in the genome is irreversible and of known polarity. These attributes make analysis of SINEs as phylogenetic characters an essentially homoplasy-free affair. Alu elements are primate-specific SINEs that make up a large portion of the human genome and are also widespread in other primates. Using a combination wet-bench and computational approach we recovered 190 Alu insertions, 183 of which are specific to the genomes of nine New World primates. We used these loci to investigate branching order and have produced a cladogram that supports a sister relationship between Atelidae (spider, woolly, and howler monkeys) and Cebidae (marmosets, tamarins, and owl monkeys) and then the joining of this two family clade to Pitheciidae (titi and saki monkeys). The data support these relationships with a homoplasy index of 0.00. In this study, we report one of the largest applications of SINE elements to phylogenetic analysis to date, and the results provide a robust molecular phylogeny for platyrrhine primates. (c) 2004 Elsevier Inc. All rights reserved.