RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells.
RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells.
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DOI：
10.1007/978-1-4939-7108-4_13
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Ables ET
中科院分区：
文献类型：
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作者：
Blake AJ;Finger DS;Hardy VL;Ables ET
阐明促进干细胞维持的分子机制的完整库需要用于鉴定和表征干细胞在其天然环境中的基因功能的复杂技术。果蝇（Drosophila melanogaster）卵巢生殖系干细胞是研究体内驱动干细胞功能的复杂分子机制的理想模型。各种新的遗传工具使RNAi成为传统遗传突变体的有益补充，用于研究指导卵巢生殖系干细胞功能的分子机制。在这里，我们提供了一个详细的指南，使用靶向RNAi敲低发现基因功能的卵巢生殖系干细胞及其后代。
Elucidating the full repertoire of molecular mechanisms that promote stem cell maintenance requires sophisticated techniques for identifying and characterizing gene function in stem cells in their native environment. Ovarian germline stem cells in the fruit fly, Drosophila melanogaster, are an ideal model to study the complex molecular mechanisms driving stem cell function in vivo. A variety of new genetic tools make RNAi a useful complement to traditional genetic mutants for the investigation of the molecular mechanisms guiding ovarian germline stem cell function. Here, we provide a detailed guide for using targeted RNAi knockdown for the discovery of gene function in ovarian germline stem cells and their progeny.