Lentiviral delivery of a shRNA sequence analogous to miR-4319/miR-125-5p induces apoptosis in NSCLC cells by arresting G2/M phase
Lentiviral delivery of a shRNA sequence analogous to miR-4319/miR-125-5p induces apoptosis in NSCLC cells by arresting G2/M phase
复制标题
慢病毒递送类似于 miR-4319/miR-125-5p 的 shRNA 序列通过阻滞 G2/M 期诱导 NSCLC 细胞凋亡
DOI：
10.1002/jcb.28676
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发表时间：
2019
影响因子：
4
通讯作者：
Hua Zichun
中科院分区：
文献类型：
--
作者：
Xu Xuebo;Lai Yueyang;Zhou Wenzhao;Hua Zichun
In this study, we explored the therapeutic potential of microRNA (miR) analogs against non–small‐cell lung cancer (NSCLC) using lentiviral delivery of short hairpin RNA (shRNA). By using A549 as a model cell line, we used analogs and mimics of miR‐4319/miR‐125‐5p to target the tumorigenic RAF1 gene. Lentiviral vectors carrying shRNA of a highly efficient miRNA analog of miR‐4319/miR‐125‐5p, Analog2, were constructed to infect A549 cells. Our results showed that, compared with the noncancerous bronchial epithelial cell line 16HBE, lentivirus delivering Analog2 shRNA induced significant G2/M arrest and subsequent apoptosis in A549 cells, but not in 16HBE cells. Western blot analysis revealed that key factors regulating cell cycle were downregulated following RAF1 inhibition. In vivo xenograft experiments showed that lentivirus carrying Analog2 shRNA markedly decreased tumor size. Therefore, lentiviral delivery of Analog2 shRNA is a valid RNA interference‐based treatment against NSCLC with high potency and specificity.
