STEROID CONCENTRATIONS IN ISOLATED THECA AND GRANULOSA LAYERS OF PREOVULATORY FOLLICLES DURING THE OVULATORY CYCLE OF THE DOMESTIC HEN
STEROID CONCENTRATIONS IN ISOLATED THECA AND GRANULOSA LAYERS OF PREOVULATORY FOLLICLES DURING THE OVULATORY CYCLE OF THE DOMESTIC HEN
复制标题
DOI：
10.1095/biolreprod29.2.326
复制
发表时间：
1983-01-01
影响因子：
3.6
通讯作者：
CALVO, FO
中科院分区：
文献类型：
--
作者：
BAHR, JM;WANG, SC;CALVO, FO
孕酮(P4)、睾酮(T)和雌二醇-17β浓度。(E_2)在家母鸡5个排卵前卵泡的离体膜和颗粒层中进行测定。分别于预期排卵前24、18、12、6、2 h摘除最大卵泡(F1)、第二大卵泡(F2)、第三大卵泡(F3)、第四大卵泡(F4)和第五大卵泡(F5)。分离卵泡膜层和颗粒层，测定P4、T、E_2和蛋白质浓度。在排卵前的5个排卵周期中，颗粒层和膜层的蛋白质浓度分别增加了5倍和15倍。随着卵泡接近排卵，卵泡膜层的E_2浓度呈线性下降，其中以排卵周期的24~18h下降最为显著(P<；0.001)。在F3和F4卵膜层中，排卵周期6h时，E_2显著升高(P<；0.005)。膜层和颗粒层的T浓度波动相似。在排卵周期的6h，F2、F3和F4卵泡层的T均显著升高(P<；0.005)，而在排卵周期的2 h，卵膜层的T显著降低(P<；0.05)。卵泡成熟过程中颗粒层P4浓度逐渐升高，排卵周期18~12h F2和F1颗粒层P4浓度升高幅度最大。卵泡成熟时，颗粒层的类固醇激素生成活性增加，而卵泡膜层的类固醇激素生成活性降低；卵泡成熟过程中，卵泡膜层中的E_2浓度逐渐降低，而颗粒层中的P_4浓度逐渐升高。
The concentrations of progesterone (P4), testosterone (T) and estradiol-17.beta. (E2) were measured in isolated theca and granulosa layers of the 5 preovulatory follicles of the domestic hen. The largest follicle (F1), the 2nd largest (F2), 3rd largest (F3), 4th largest (F4) and 5th largest (F5) follicles were removed at 24, 18, 12, 6 and 2 h before the expected ovulation. Theca and granulosa layers were isolated and P4, T, E2 and protein concentrations determined. Protein concentrations of the granulosa and theca layers increased 5- and 15-fold, respectively, during the 5 ovulatory cycles prior to ovulation. As the follicle approached ovulation, there was a linear decrease in E2 concentration of the theca layer with the most significant decrease (P < 0.001) occurring between 24 and 18 h of the ovulatory cycle. In the F3 and F4 theca layers, there was a significant increase (P < 0.005) in E2 at 6 h of the ovulatory cycle. Fluctuations in T concentrations in theca and granulosa layers were similar. There was a significant increase (P < 0.05) in T in both layers of the F2, F3 and F4 follicles at 6 h followed by a decrease (P < 0.005) in the theca layers at 2 h of the ovulatory cycle. The P4 concentration of the granulosa layer increased gradually during follicular maturation with the greatest increase occurring in the F2 and F1 granulosa layers between 18 and 12 h of the ovulatory cycle. The steroidogenic activity of the granulosa layer increases while that of the theca layer decreases during follicular maturation, and there is a gradual decrease in the concentration of E2 in the theca layer while there is an increase in P4 in the granulosa layer during follicular maturation.