From stationary to instationary metabolic flux analysis.
From stationary to instationary metabolic flux analysis.
复制标题
从静态到静态代谢通量分析。
DOI：
10.1007/b98921
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
K. Nöh
中科院分区：
文献类型：
--
作者：
W. Wiechert;K. Nöh
使用 13C 标记底物进行代谢通量分析是代谢工程的重要工具。尽管代谢流分析现在已经发展了十多年，但仍然没有达到其应用的极限。当前的发展一方面旨在扩展到固定工业生产条件，例如分批和补料分批发酵，另一方面旨在小型化和高通量分析。在这两种情况下，减少标记实验的持续时间是人们最感兴趣的。因此，现在人们对标记实验期间 13C 富集的瞬时瞬态产生兴趣。本文介绍了静态代谢通量分析领域的一些最新进展，并使用一些模拟示例讨论了一些关键方面和局限性。
Metabolic flux analysis using 13C labeled substrates is an important tool for metabolic engineering. Although it has now been evolving for more than ten years, metabolic flux analysis has still not reached the limits of its application. Current developments aim at extending to instationary industrial production conditions like batch and fed-batch fermentations on the one hand, and at miniaturization and high throughput flux analysis on the other. In both cases, reducing the duration of the labeling experiment is of major interest. For that reason, there is now interest in the instationary transient of the 13C enrichment during a labeling experiment. This paper presents some recent developments in the field of instationary metabolic flux analysis and discusses some critical aspects and limitations using some simulation examples.