Profiling the Humoral Immune Response of Acute and Chronic Q Fever by Protein Microarray
Profiling the Humoral Immune Response of Acute and Chronic Q Fever by Protein Microarray
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DOI：
10.1074/mcp.m110.006304
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发表时间：
2011-10-01
影响因子：
7
通讯作者：
Felgner, Philip L.
中科院分区：
文献类型：
--
作者：
Vigil, Adam;Chen, Chen;Felgner, Philip L.
使用全面的蛋白质微阵列进行抗原分析是表征对感染性病原体的体液免疫应答的有力工具。贝氏柯克斯体是CDC分类的B类生物恐怖感染剂，在世界范围内分布。为了评估急性和慢性Q热患者的抗体谱，我们构建了一个蛋白质微阵列，其中包含Q热病原体贝氏柯克斯体93%的蛋白质组。本文报告了25例急性Q热患者的IgG和IgM血清反应性。我们发现，感染的早期和晚期时间点都具有非常一致的IgM和IgG反应谱，其中有限数量的蛋白质经历增加或减少的血清反应性。我们还探测了大量的急性和慢性Q热患者样本，并确定了可以区分这两种疾病状态的血清学标志物。在这项比较分析中，我们确认了急性感染的许多IgG生物标志物的身份，鉴定了急性和慢性感染的新型IgG生物标志物，并首次分析了急性和慢性Q热的IgM抗体库。利用这些结果，我们能够设计出一种可以区分急性和慢性Q热的测试。这些结果也提供了一个独特的观点同种型开关，并证明了实用的蛋白质微阵列，同时检查动态体液免疫反应对成千上万的蛋白质从大量的患者。本文提出的结果确定了用于开发基于重组蛋白的诊断和亚单位疫苗的新型血清反应性抗原，并提供了对抗体应答发展的深入了解。Molecular & Cellular Proteomics 10：10.1074/mcp. M110.006304，1-12，2011.
Antigen profiling using comprehensive protein microarrays is a powerful tool for characterizing the humoral immune response to infectious pathogens. Coxiella burnetii is a CDC category B bioterrorist infectious agent with worldwide distribution. In order to assess the antibody repertoire of acute and chronic Q fever patients we have constructed a protein microarray containing 93% of the proteome of Coxiella burnetii, the causative agent of Q fever. Here we report the profile of the IgG and IgM seroreactivity in 25 acute Q fever patients in longitudinal samples. We found that both early and late time points of infection have a very consistent repertoire of IgM and IgG response, with a limited number of proteins undergoing increasing or decreasing seroreactivity. We also probed a large collection of acute and chronic Q fever patient samples and identified serological markers that can differentiate between the two disease states. In this comparative analysis we confirmed the identity of numerous IgG biomarkers of acute infection, identified novel IgG biomarkers for acute and chronic infections, and profiled for the first time the IgM antibody repertoire for both acute and chronic Q fever. Using these results we were able to devise a test that can distinguish acute from chronic Q fever. These results also provide a unique perspective on isotype switch and demonstrate the utility of protein microarrays for simultaneously examining the dynamic humoral immune response against thousands of proteins from a large number of patients. The results presented here identify novel seroreactive antigens for the development of recombinant protein-based diagnostics and subunit vaccines, and provide insight into the development of the antibody response. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.006304, 1-12, 2011.