The H9C2 cell line and primary neonatal cardiomyocyte cells show similar hypertrophic responses in vitro
The H9C2 cell line and primary neonatal cardiomyocyte cells show similar hypertrophic responses in vitro
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DOI：
10.1007/s11626-010-9368-1
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发表时间：
2011-02-01
影响因子：
2.1
通讯作者：
Arthur, Helen M.
中科院分区：
文献类型：
--
作者：
Watkins, Sarah J.;Borthwick, Gillian M.;Arthur, Helen M.
心脏肥厚是心衰及相关患者发病率和死亡率的主要危险因素。研究心脏肥厚过程中发生的异常分子过程，使用新生大鼠心脏的原代心肌细胞作为标准的体外实验系统。此外，一些研究利用H9C2大鼠成心肌细胞系，其优点是作为一种无动物替代；然而，H9C2细胞在多大程度上能够准确模拟原代心肌细胞的肥厚反应尚未完全确定。为了解决这一局限性，我们直接比较了H9C2细胞与大鼠新生心肌细胞在肥厚因子刺激后的肥厚反应。体外培养大鼠新生心肌细胞和H9C2细胞，并给予血管紧张素II和内皮素-1促进增生性反应。使用显微镜和实时rtPCR直接比较了两种细胞类型中细胞足迹增加、细胞骨架重排和胎儿心脏基因诱导。H9C2细胞表现出与原代心肌细胞几乎相同的肥厚反应。这一发现验证了H9C2细胞作为心脏肥大体外研究模型的重要性，并支持了目前人类心肌细胞系在心脏发育和疾病的前瞻性分子研究中的工作。
Cardiac hypertrophy is a major risk factor for heart failure and associated patient morbidity and mortality. Research investigating the aberrant molecular processes that occur during cardiac hypertrophy uses primary cardiomyocytes from neonatal rat hearts as the standard experimental in vitro system. In addition, some studies make use of the H9C2 rat cardiomyoblast cell line, which has the advantage of being an animal-free alternative; however, the extent to which H9C2 cells can accurately mimic the hypertrophic responses of primary cardiac myocytes has not yet been fully established. To address this limitation, we have directly compared the hypertrophic responses of H9C2 cells with those of primary rat neonatal cardiomyocytes following stimulation with hypertrophic factors. Primary rat neonatal cardiomyocytes and H9C2 cells were cultured in vitro and treated with angiotensin II and endothelin-1 to promote hypertrophic responses. An increase in cellular footprint combined with rearrangement of cytoskeleton and induction of foetal heart genes were directly compared in both cell types using microscopy and real-time rtPCR. H9C2 cells showed almost identical hypertrophic responses to those observed in primary cardiomyocytes. This finding validates the importance of H9C2 cells as a model for in vitro studies of cardiac hypertrophy and supports current work with human cardiomyocyte cell lines for prospective molecular studies in heart development and disease.