Anti-inflammatory function of ginsenoside Rg1 on alcoholic hepatitis through glucocorticoid receptor related nuclear factor-kappa B pathway
Anti-inflammatory function of ginsenoside Rg1 on alcoholic hepatitis through glucocorticoid receptor related nuclear factor-kappa B pathway
复制标题
人参皂苷Rg1通过糖皮质激素受体相关核因子-κB通路抗酒精性肝炎的作用
DOI：
10.1016/j.jep.2015.07.020
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发表时间：
2015-09-15
影响因子：
5.4
通讯作者：
Chen, Naihong
中科院分区：
文献类型：
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作者：
Gao, Yan;Chu, Shifeng;Chen, Naihong
民族药理学相关性：人参是人参C.A.的干燥根。梅耶自古以来，人参在中国甚至韩国和日本等东方国家都被用作一种治疗药物或补品。人参的药理活性化学成分及其提取物均为三萜皂苷类混合物，统称人参皂苷。研究目的：基于以往的实验结果和对酒精性肝炎的认识，本研究的主要目的是探讨Rg 1是否有益于酒精性肝损伤的动物模型，并探讨其可能的作用机制。材料与方法：C57 BL/6小鼠连续9天分别灌胃给予人参皂甙Rg 1（10、20和40 mg/kg）或地塞米松（1 mg/kg）后1 h，灌胃6 g/kg酒精。进行生化分析和肝碎片进行处理显微镜，免疫组化和westernblot analysis.Results：根据我们的数据，人参皂甙1治疗显着扭转了高死亡率引起的酒精消费，也减轻了肝损伤的血清参数的下降证明。与此同时，酒精组的组织学和超微结构分析显示肝细胞损伤，但Rg 1治疗组恢复。Rg 1在酒精中毒小鼠肝脏中也抑制了过度产生的炎性细胞因子。结论：Rg 1可能通过抑制NF-κ B的表达，促进GR的表达，从而抑制酒精性肝炎的炎症反应。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Ginseng is the dried root of Panax ginseng C.A. Mayer. Since ancient times, ginseng has been used as one kind of treatment drug or tonic in China and even other eastern countries like Korea and Japan. Pharmacological active chemical ingredients and its extract of ginseng are a mixture of triterpenoid saponins, collectively called ginsenosides. Among them, ginsenoside Rg1 is the most pharmacological active one.Aim of the study: Based on prior experimental results and the understanding of alcoholic hepatitis, the major aim of this study is to investigate whether Rg1 is beneficial in a rodent model mimic alcoholic hepatic injury associated with binge drinking and explore the underlying possible mechanisms. Materials and methods: C57BL/6 mice were given oral consumption of 6 g/kg alcohol 1 h after treated with Rg1 (10, 20 and 40 mg/kg) or dexamethasone (1 mg/kg) for 9 consecutive days. Biochemical analyses were performed and liver fragments were processed for microscopy, immunohistochemistry and western blot analysis.Results: According to our data, Rg1 treatment significantly reversed the high mortality rate induced by alcohol consumption and also alleviated liver impairment as evidenced by the decrease of serum parameters. Meanwhile, histological and ultrastructural analysis of alcoholic groups showed hepatocellular impairment but restored in Rg1-treated groups. Overproductive inflammatory cytokines were also suppressed by Rg1 in alcohol-intoxicated mouse livers. In addition, changes of GR related NF-kappa B pathway, including phospho-I kappa B-alpha, were also modulated to normal levels.Conclusion: This study demonstrates that Rg1 might promote GR mediating the repression of NF-kappa B and inhibit the inflammatory reactions in alcoholic hepatitis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.