Dye extract of calyces of Hibiscus sabdariffa has photodynamic antibacterial activity: A prospect for sunlight‐driven fresh produce sanitation
Dye extract of calyces of Hibiscus sabdariffa has photodynamic antibacterial activity: A prospect for sunlight‐driven fresh produce sanitation
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DOI：
10.1002/fsn3.1580
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发表时间：
2020-04
影响因子：
3.9
通讯作者：
Hussaini Majiya;A. Galstyan
中科院分区：
文献类型：
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作者：
Hussaini Majiya;A. Galstyan
摘要在传统的卫生方法不可用的地方，新鲜农产品的光动力卫生可以帮助减少腐败和疾病传播，而阳光是免费的。本研究评价了玫瑰茄花萼染料提取物的光稳定性和光动力抗菌活性。染料浸提液在水中非常耐光，但在醋酸盐-HCl缓冲液（pH 4.6）、磷酸盐缓冲盐水（pH 7.2）和tris碱-HCl缓冲液（pH 8.6）中可漂白。光稳定性与染料提取物的光动力学抗菌活性相关。浓度为0.0625 mg/ml的甲醇和水染料浸提液在10 mW/cm 2的可见光暴露下或在无光暴露的黑暗中均能在2 min内完全灭活枯草芽孢杆菌（减少8.5 log CFU/ml）。在10 mW/cm ~ 2光照20 min条件下，用1 mg/ml甲醇和水染料浸提液分别对大肠杆菌进行4.8 log CFU/ml和2.2 log CFU/ml的降解。sabdariffa甚至在水中，而不需要加热的能量，以及染料提取物对于新鲜产品卫生的适用性。黄藤花萼染料提取物。sabdariffa具有光动力和非光动力抗菌活性，可用于开发廉价、可持续和环境友好的低技术阳光驱动的新鲜农产品卫生系统。
Abstract Photodynamic sanitation of fresh produce could help reduce spoilage and disease transmissions where conventional methods of sanitation are not available, and sunlight is available for free. In this study, we evaluated the photostability and photodynamic antibacterial activity of the dye extracts of calyces of Hibiscus sabdariffa. The dye extracts were very photostable in water but bleached in acetate‐HCl buffer (pH 4.6), phosphate buffer saline (pH 7.2), and tris base‐HCl buffer (pH 8.6). The photostability correlated with the photodynamic antibacterial activity of the dye extracts. Both the methanol and water dye extracts at the concentration of 0.0625 mg/ml caused complete inactivation of Bacillus subtilis (reductions of 8.5 log CFU/ml) within 2 min either with the visible light exposure at 10 mW/cm2 or in the dark without the light exposure. Reductions of 4.8 log CFU/ml and 2.2 log CFU/ml of Escherichia coli were observed when 1 mg/ml of methanol and water dye extracts were used, respectively, in water with the light exposure at 10 mW/cm2 for 20 min. Discussions are included about the ease of the dye extractions of the calyces of H. sabdariffa even in water without the need of energy for heating and the suitability of the dye extracts for the fresh produce sanitation. Dye extract of calyces of H. sabdariffa has photodynamic and nonphotodynamic antibacterial activity which could be exploited for the development of a low‐tech sunlight‐driven fresh produce sanitation system that is cheap, sustainable, and environmentally friendly.