Acid Hydrolysates of Casein to Replace Peptone in the Preparation of Bacteriological Media
Acid Hydrolysates of Casein to Replace Peptone in the Preparation of Bacteriological Media
复制标题
酪蛋白酸水解物替代蛋白胨制备细菌培养基
作者：
J. Mueller;E. R. Johnson
病原菌生长所用的传统培养基用“蛋白胨”配制，往往呈现出某些明显的弊端。一方面，它的存在严重干扰了细菌生长的各种产物的纯形式的分离，毒素，特定的碳水化合物等。同样，从含有蛋白胨的介质中制备用于预防的类毒素并不是没有风险的，因为有记录的例子，即使是在希克试验中作为稀释剂注射的微量也有严重反应。另一个缺点是大量的蛋白调之间的差异，即使是同一品牌的，这是不可避免的，由于生产方法的原因。
似乎太多时候，这些商业产品被认为是化学实体，“蛋白内酯”这个词被用来暗示一个概念，即使不是像“盐”或“葡萄糖”那样明确，也几乎是这样。
The traditional use of “peptone” for the preparation of culture media for the growth of pathogenic bacteria often presents certain obvious disadvantages. On the one hand, its presence seriously interferes with the isolation in pure form of the various products of bacterial growth,—toxins, specific carbohydrates, etc. Again, the preparation of toxoids for prophylactic use from a medium which contains peptone is not without risk, for there are instances on record of severe reactions even to the minute amounts injected when it is employed as a diluent in the Schick test. An additional drawback lies in the variation between lots of peptone, even of the same brand, which is inevitable and due to the methods of production.
It appears that too often these commercial products have been considered as chemical entities, the word “peptone” being used to imply a conception almost, if not quite as definite as “salt” or “glucose.”