Enzymatic oxidation and reduction of retinal by mouse epidermis.
Enzymatic oxidation and reduction of retinal by mouse epidermis.
复制标题
小鼠表皮对视网膜的酶促氧化和还原。
DOI：
10.1016/0006-291x(80)90572-0
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
R. Boutwell
中科院分区：
文献类型：
--
作者：
G. S. Kishore;R. Boutwell
证据是小鼠表皮氧化视网膜的细胞溶质酶的作用，最佳pH值约为8.5的能力。添加NAD或FAD可使酶活性略有提高。该反应表现出米氏动力学，对视黄醛的km为1.627 × 10− 4 M。小鼠表皮细胞液也能降低视网膜。该酶的最适pH为6.0，通过加入还原的NAD或NADP，未透析酶的活性增强5- 7倍。这些酶的活性在表皮中的可能作用进行了讨论。
Evidence is presented for the ability of mouse epidermis to oxidize retinal by the action of a cytosolic enzyme with a pH optimum of about 8.5. The enzyme activity was slightly enhanced by the addition of either NAD or FAD. The reaction exhibited Michaelis-Menton kinetics with a km of 1.627 × 10−4M for retinal. Mouse epidermal cytosol was also capable of reducing retinal. This enzyme had an optimum pH of 6.0 and the activity of the undialyzed enzyme was enhanced 5- to 7-fold by the addition of reduced NAD or NADP. Possible roles of these enzymic activities in epidermis are discussed.