The cholesterol side-chain cleavage system of bovine adrenal cortex.
The cholesterol side-chain cleavage system of bovine adrenal cortex.
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DOI：
10.1111/j.1432-1033.1967.tb00136.x
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发表时间：
1967-10
期刊：
影响因子：
--
通讯作者：
Evan R. Simpson;George S. Boyd
中科院分区：
文献类型：
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作者：
Evan R. Simpson;George S. Boyd
1用超声提取的线粒体提取液作为酶活性来源，研究了牛肾上腺皮质胆固醇侧链的裂解系统。2已发现一氧化碳对该系统有抑制作用，这种抑制作用与一氧化碳和氧气对酶切位点的竞争一致。3一氧化碳的抑制作用被光逆转，最有效的波长是450mμ。这些结果表明，含有血红素的蛋白质‘P-450’参与了胆固醇侧链裂解系统中的氧结合部位。为了分离胆固醇和孕烯醇酮之间的中间体，已经进行了4次尝试。未发现20α-羟基胆固醇和20α，22-二羟基胆固醇。
1 The cholesterol side-chain cleavage system of bovine adrenal cortex has been studied using extracts of sonicated mitochondria as source of enzymic activity. 2 The system has been found to be inhibited by carbon monoxide, this inhibition being consistent with competition between carbon monoxide and oxygen for an enzymic site. 3 The inhibition by carbon monoxide is reversed by light, the most effective wavelength being 450 mμ. These results suggest the involvement of a haem-containing protein, ‘P-450’ as the oxygen-binding site in the cholesterol side-chain cleavage system. 4 Attempts have been made to isolate intermediates between cholesterol and pregnenolone. Neither 20α-hydroxycholesterol nor 20α,22-dihydroxycholesterol have been found.