Measuring mitochondrial function in intact cardiac myocytes.
Measuring mitochondrial function in intact cardiac myocytes.
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DOI：
10.1016/j.yjmcc.2011.08.030
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发表时间：
2012-01
影响因子：
5
通讯作者：
Blatter LA
中科院分区：
文献类型：
--
作者：
Dedkova EN;Blatter LA
线粒体参与的细胞功能超出了这些细胞器作为细胞发电厂的传统作用。线粒体与多种人类疾病有关，包括心脏功能障碍，并在衰老过程中发挥作用。我们对线粒体的了解的许多方面都源于对分离的细胞器进行的研究。它们相对难以接近，给研究自然环境（完整细胞的细胞质）中的线粒体带来了实验困难，并阻碍了对线粒体过多功能的全面了解。在这里，我们回顾目前研究完整心肌细胞线粒体功能的可用方法。这些方法主要使用不同口味的荧光染料和基因编码的荧光蛋白以及高分辨率成像技术。我们回顾了研究线粒体形态、线粒体膜电位、Ca2+ 和 Na+ 信号传导、线粒体 pH 调节、氧化还原状态和 ROS 产生、NO 信号传导、耗氧量、ATP 产生和线粒体通透性转换孔活性的方法。在适当的情况下，我们通过对分离的线粒体、透化细胞和整个心脏进行的开创性研究来补充对完整心肌细胞的综述。
Mitochondria are involved in cellular functions that go beyond the traditional role of these organelles as the power plants of the cell. Mitochondria have been implicated in several human diseases, including cardiac dysfunction, and play a role in the aging process. Many aspects of our knowledge of mitochondria stem from studies performed on the isolated organelle. Their relative inaccessibility imposes experimental difficulties to study mitochondria in their natural environment – the cytosol of intact cells – and has hampered a comprehensive understanding of the plethora of mitochondrial functions. Here we review currently available methods to study mitochondrial function in intact cardiomyocytes. These methods primarily use different flavors of fluorescent dyes and genetically encoded fluorescent proteins in conjunction with high-resolution imaging techniques. We review methods to study mitochondrial morphology, mitochondrial membrane potential, Ca2+ and Na+ signaling, mitochondrial pH regulation, redox state and ROS production, NO signaling, oxygen consumption, ATP generation and the activity of the mitochondrial permeability transition pore. Where appropriate we complement this review on intact myocytes with seminal studies that were performed on isolated mitochondria, permeabilized cells, and in whole hearts.
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缺血期间增强的Na+/H+交换会损害线粒体生物能和心肌功能。
影响因子：
3
作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
Blatter, Lothar A.