Inhibitory effect of Lactobacillus acidophilus on Helicobacter hepaticus in vitro
Inhibitory effect of Lactobacillus acidophilus on Helicobacter hepaticus in vitro
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DOI：
10.1007/s11274-012-1203-2
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发表时间：
2013-01
影响因子：
4.1
通讯作者：
Man-hua Zhang;Haiyang Zhang;Y. Li;Wen-sai Qi;Xu Wang;Jiang-bing Wang
中科院分区：
文献类型：
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作者：
Man-hua Zhang;Haiyang Zhang;Y. Li;Wen-sai Qi;Xu Wang;Jiang-bing Wang
肝幽门螺杆菌和幽门螺杆菌都属于幽门螺杆菌种。嗜酸乳杆菌L4和L6菌株对h。幽门。基于这一现象，我们旨在研究l的抑制作用。acidophilusonH。hepaticus。标准h和隔离h。在37°C、l存在的微嗜氧条件下培养肝菌。嗜酸上清，或乳酸。在固体培养基上测定了抑菌带的直径。在液体培养中，测定细胞浓度，用酚红染色法测定脲酶活性。16株l。从人粪便中分离的嗜酸杆菌（命名为L1-L16）显示抗h。hepaticuseffects。其中L4和L6对h的影响最为明显。hepaticusinhibition。TheL。与乳酸对照相比，L4和L6的嗜酸菌上清液显著增加了抑菌带直径（P< 0.05）。l的抑制作用。嗜酸菌上清液与溶液pH值无关（P < 0.05）。此外，在液体培养中，L。嗜酸菌上清液显著降低细胞生长速率和酶活性。肝细胞呈时间依赖性（与乳酸对照组相比P< 0.05）。标准株与分离株间无明显差异。hepaticus (P > 0.05)。我们的结果表明，l。嗜酸乳杆菌能降低h。肝毒素的体外抑制作用与pH值无关。这为开发预防和治疗h的新方法提供了证据。hepaticusinfection。
Helicobacter hepaticusandHelicobacter pyloriboth belong to Helicobacter species. Strains ofLactobacillus acidophilus, including L4 and L6, have shown significant inhibitory effects onH. pylori. Based on this phenomenon, we aim to investigate the inhibitory effect ofL. acidophilusonH. hepaticus. Both standard and isolatedH. hepaticusstrains were grown under microaerophilic conditions at 37 °C in the presence ofL. acidophilussupernatant, or lactic acid. The diameters of the inhibition zones were measured on the solid culture media. In liquid culture, the cell concentrations were measured and the urease activity was determined by phenol red staining. Sixteen strains ofL. acidophilusisolated from human feces (named as L1–L16) showed anti-H. hepaticuseffects. Two of them (L4 and L6) exhibited the most apparent effects onH. hepaticusinhibition. TheL. acidophilussupernatant of L4 and L6 significantly increased the diameters of the inhibition zones compared with that of the lactic acid control (P< 0.05). The inhibitory role ofL. acidophilussupernatant was independent of the pH value of solution (P> 0.05). Moreover, in liquid culture,L. acidophilussupernatant significantly reduced the cell growth rate and the urease activity ofH. hepaticuscells in a time-dependent pattern (P< 0.05 compared with lactic acid control). No obvious difference was observed between the standard and isolated strain ofH. hepaticus(P> 0.05). Our results indicate thatL. acidophiluscan decrease the viability and urease activity ofH. hepaticusin vitro and this inhibition is independent of pH levels. This provides evidence for developing novel approaches for the prevention and treatment ofH. hepaticusinfection.