Near-infrared spectrophotometry: a new dimension in clinical chemistry.
Near-infrared spectrophotometry: a new dimension in clinical chemistry.
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DOI：
10.1093/clinchem/38.9.1623
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发表时间：
1992-09
影响因子：
9.3
通讯作者：
Jeffrey W. Hall;Alan
中科院分区：
文献类型：
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作者：
Jeffrey W. Hall;Alan
近红外（NIR）光谱区（700-2500 nm）是化学信息的丰富来源，其形式为基本红外吸收和低能电子跃迁的泛音和组合带。这一区域最初被认为过于复杂，难以解释，因此没有得到充分利用。化学计量学技术的进步可以从高度重叠的复杂光谱中提取大量的化学信息，这使得近红外分光光度法（NIRS）在食品，农业，制药，化学和聚合物工业中得到广泛应用。近红外光谱在临床实验室测量中的应用尚处于起步阶段。近红外光谱是一种简单、快速、无损的技术，能够提供临床相关的生物样品分析，其精密度和准确度与用于推导近红外光谱模型的方法相当。分析可以在很少或没有样品制备和没有试剂的情况下进行。在任何特定情况下，NIRS的成功取决于样品基质的复杂性、组分的相对NIR吸收率以及所选择的波长和回归技术。我们描述的一般方法，数据采集，校准和分析，使用血清蛋白，甘油三酯和葡萄糖作为例子。
The near-infrared (NIR) spectral region (700-2500 nm) is a fertile source of chemical information in the form of overtone and combination bands of the fundamental infrared absorptions and low-energy electronic transitions. This region was initially perceived as being too complex for interpretation and consequently was poorly utilized. Advances in chemometric techniques that can extract massive amounts of chemical information from the highly overlapped, complex spectra have led to extensive use of NIR spectrophotometry (NIRS) in the food, agriculture, pharmaceutical, chemical, and polymer industries. The application of NIRS in clinical laboratory measurements is still in its infancy. NIRS is a simple, quick, nondestructive technique capable of providing clinically relevant analyses of biological samples with precision and accuracy comparable with the method used to derive the NIRS models. Analyses can be performed with little or no sample preparation and no reagents. The success of NIRS in any particular case is determined by the complexity of the sample matrix, relative NIR absorptivities of the constituents, and the wavelengths and regression technique chosen. We describe the general approach to data acquisition, calibration, and analysis, using serum proteins, triglycerides, and glucose as examples.