ON THE ROLE OF STICKINESS OF TUMOR CELLS IN THE FORMATION OF METASTASES.
ON THE ROLE OF STICKINESS OF TUMOR CELLS IN THE FORMATION OF METASTASES.
复制标题
关于肿瘤细胞粘性在转移形成中的作用。
作者：
K. Kojima;I. Sakai
总结使用三种大鼠腹水肝癌细胞株AH 13、AH 130和AH 7974进行了肿瘤细胞对玻璃表面粘附性的比较研究。通过计算在用盐溶液洗涤后粘附在计算板的玻璃表面上的细胞的百分比来测量粘性。3株菌的细胞粘性差异显著。AH 13表现出最活跃的粘性，AH 130中等，AH 7974最不活跃。这些结果表明，在上述肿瘤中，细胞粘性与细胞之间的粘附性呈负相关。当静脉内接种这三种肿瘤菌株时，就细胞粘性而言最活跃的菌株引起肺中最大数量的转移性结节。每种肿瘤的腹膜内接种也获得了相同的肺转移频率结果。转移频率和细胞粘性之间的这种相关性表明，细胞粘性可能是负责肿瘤细胞通过血管转移扩散的细胞表面的重要性质。为了研究粘性的性质，使用埃利希腹水癌、AH 13和吉田肉瘤进行体外肿瘤细胞的化学处理。结果发现，用硫酸鱼精蛋白和胰蛋白酶处理的肿瘤细胞的粘性降低，而用吐温-80处理的粘性增加。用聚乙烯硫酸酯、脱氧胆酸钠、氯化钙和versenate二钠不能观察到对粘性的显著影响。在酸性培养基中，细胞粘性显著降低，而在碱性培养基中，细胞粘性增加。在此基础上，对肿瘤细胞与玻璃的粘附性进行了讨论。
Summary A comparative study of stickiness of tumor cells to glass surfaces was undertaken with the use of three strains of rat ascites hepatoma, AH 13, AH 130, and AH 7974. Stickiness was measured by calculating the percentage of cells clinging to a glass surface of the calculating plate after it was washed with salt solution. A significant difference was observed in the cell stickiness among these three strains. AH 13 showed the most active stickiness, AH 130 moderate, and AH 7974 the least active. These results indicate that cell stickiness is inversely correlated with the adhesiveness between cells in the above tumors. When these three tumor strains were inoculated intravenously, the most active strain with respect to cell stickiness caused the largest number of metastatic nodules in the lung. The same result in frequency of lung metastasis was also obtained with intraperitoneal inoculation of each tumor. Such a correlation between metastatic frequency and cell stickiness suggests that the cell stickiness may be an important property of the cell surface responsible for metastatic spread of tumor cells via blood vessels. In order to study the nature of stickiness, chemical treatments of tumor cells in vitro were undertaken with the use of Ehrlich ascites carcinoma, AH 13, and Yoshida sarcoma. It was found that the stickiness of tumor cells was decreased by treatment with protamine sulfate and trypsin, whereas stickiness was increased by treatment with Tween-80. Significant effects on stickiness could not be observed with polyvinyl sulfate, sodium deoxycholate, calcium chloride, and disodium versenate. Furthermore, in acid medium, cell stickiness was significantly decreased, while in alkaline medium it was increased. Based on these results, stickiness of tumor cells to glass was discussed.