Discovery of unique single nucleotide polymorphisms in rice in response to high nighttime temperature stress using a hybrid sequencing strategy
Discovery of unique single nucleotide polymorphisms in rice in response to high nighttime temperature stress using a hybrid sequencing strategy
复制标题
使用混合测序策略发现水稻响应夜间高温胁迫的独特单核苷酸多态性
DOI：
10.1016/j.envexpbot.2019.02.005
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发表时间：
2019
影响因子：
5.7
通讯作者：
Jiang Lin Liao
中科院分区：
文献类型：
--
作者：
Hui Wen Zhou;Hong Yu Zhang;Zhao Hai Wang;Chao He;Li Li You;Dong Hui Fu;Jian Bo Song;Ying Jin Huang;Jiang Lin Liao
与全球变暖相关的温度升高，特别是在夜间，不利于水稻籽粒灌浆，最终导致谷粒重量损失。然而，与夜间高温胁迫下水稻籽粒失重相关的分子机制还知之甚少。为筛选与夜间高温胁迫相关的基因和单核苷酸多态性（SNPs），采用杂交测序技术分析了水稻耐热品系（HTS）和热敏品系（HSS）在种子成熟初期短期极端高温胁迫下的差异表达基因和SNPs。最终，56个基因在HTS和HSS之间差异表达。短期极端高温胁迫后，参与光合作用，氧化和解毒谷胱甘肽的基因在HSS中上调相比，HTS，而热响应相关的转录因子基因显着上调相比，HSS。位于基因上的独特SNP过氧化物酶前体、谷胱甘肽S-转移酶GSTU 6、糖基水解酶、羧基乙烯基-羧基磷酸磷酸变位酶和醇溶蛋白前体PROLM 3存在于HTS中，但不存在于HSS中，并且在HTS和HSS之间的基因表达中显示出轻微的变化。所提出的模型表明，夜间高温增强细胞呼吸，扰乱氧化-抗氧化平衡，消耗富含能量的物质，最终导致降低粮食产量在HSS相比，高温超导。这些基因和独特的SNPs为水稻耐热品种的选育提供了遗传资源，该模型为水稻对夜间高温胁迫反应的分子基础提供了见解。
Global warming-associated increases in temperature, particularly at nighttime, are detrimental to rice grain filling, ultimately leading to losses in grain weight. However, the molecular mechanisms associated with grain weight loss in rice exposed to high nighttime temperature stress are poorly understood. To screen the genes and single nucleotide polymorphisms (SNPs) associated with high nighttime temperature stress in rice, a hybrid sequencing strategy was used to analyze the differentially expressed genes and SNPs between two rice coisogenic strains, a heat-tolerant strain (HTS) and heat-sensitive strain (HSS), following short-term extreme high nighttime temperature stress at the first stage of seed ripening. Ultimately, 56 genes were differentially expressed between HTS and HSS. After short-term extreme high nighttime temperature stress, genes involved in photosynthesis, oxidation, and detoxication by glutathione were upregulated in HSS in comparison to HTS, while that of the heat response-related transcription factor genes were significantly upregulated in HTS in comparison to HSS. Unique SNPs located on the genesperoxidase precursor,glutathione S-transferase GSTU6,glycosyl hydrolases,carboxyvinyl-carboxyphosphonate phosphorylmutase,andprolamin precursor PROLM3were present in HTS but absent from HSS and showed slight alterations in gene expression between HTS and HSS. The proposed model indicated that high nighttime temperature enhanced cellular respiration, disturbed the oxidant-antioxidant balance, and consumed energy-rich substances, ultimately leading to reduced grain yield in HSS in contrast to HTS. These genes and unique SNPs provide genetic resources for the breeding of heat-tolerant rice varieties, and the model provides insights into the molecular basis of the response of rice to high nighttime temperature stress.