Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry.
Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry.
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DOI：
10.1039/c5an02062j
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发表时间：
2016-03-07
期刊：
影响因子：
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通讯作者：
Baker ES
中科院分区：
文献类型：
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作者：
Kyle JE;Zhang X;Weitz KK;Monroe ME;Ibrahim YM;Moore RJ;Cha J;Sun X;Lovelace ES;Wagoner J;Polyak SJ;Metz TO;Dey SK;Smith RD;Burnum-Johnson KE;Baker ES
了解生物分子是如何在生命系统中产生、代谢和消除的，对于解释免疫反应和疾病病理学等过程非常重要。尽管基因组和蛋白质组研究在过去几十年中提供了大量信息，但由于分析技术的进步，人们对脂类组学的兴趣也有所增加，这些技术揭示了2型糖尿病、癌症和脂质储存疾病中脂代谢的变化。质谱学(MS)测量是目前表征脂体的主要方法，它提供了关于脂类的空间和时间组成的详细信息。然而，由于存在大量的结构和立体异构体(即不同的酰基链和双键位置)，解释脂类的生物学作用是具有挑战性的，这些异构体通常不能用现有的方法来解决。在这里，我们展示了结合液相色谱(LC)和基于结构的离子迁移率光谱(IMS)测量与MS分析来区分类脂异构体，并允许洞察生物和疾病过程。
Understanding how biological molecules are generated, metabolized and eliminated in living systems is important for interpreting processes such as immune response and disease pathology. While genomic and proteomic studies have provided vast amounts of information over the last several decades, interest in lipidomics has also grown due to improved analytical technologies revealing altered lipid metabolism in type 2 diabetes, cancer, and lipid storage disease. Mass spectrometry (MS) measurements are currently the dominant approach for characterizing the lipidome by providing detailed information on the spatial and temporal composition of lipids. However, interpreting lipids’ biological roles is challenging due to the existence of numerous structural and stereoisomers (i.e. distinct acyl chain and double-bond positions), which are often unresolvable using present approaches. Here we show that combining liquid chromatography (LC) and structurally-based ion mobility spectrometry (IMS) measurement with MS analyses distinguishes lipid isomers and allows insight into biological and disease processes.