MOLECULAR-CLONING AND EXPRESSION OF AN IL-6 SIGNAL TRANSDUCER, GP130
MOLECULAR-CLONING AND EXPRESSION OF AN IL-6 SIGNAL TRANSDUCER, GP130
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DOI：
10.1016/0092-8674(90)90411-7
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发表时间：
1990-12-21
期刊：
影响因子：
64.5
通讯作者：
KISHIMOTO, T
中科院分区：
文献类型：
--
作者：
HIBI, M;MURAKAMI, M;KISHIMOTO, T
白介素6(IL-6)信号通过与IL-6受体(IL-6-R)相关的膜糖蛋白gp130传递。人gp130基因的编码区已被克隆，它由918个氨基酸组成，具有一个跨膜区。胞外区由六个单位的纤维连接蛋白III型模块组成，该区域的一部分由大约200个氨基酸组成，具有细胞因子受体家族的典型特征。表达的gp130不能与IL-6或其他几种细胞因子结合。尽管带有IL-6-R基因的转染体主要表达低亲和力的IL-6结合位点，但与gp130共转染后，高亲和力结合位点的表达增加。这证实了gp130参与了高亲和力IL-6结合位点的形成。克隆的gp130可以与IL-6和可溶性IL-6-R的复合体结合，在小鼠IL-3依赖的生长信号中表达时，当在小鼠IL-3依赖的细胞系中表达时，可以转导生长信号。
Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein, gp130, which associates with IL-6 receptor (IL-6-R). A cDNA encoding human gp130 has been cloned, revealing that it consists of 918 amino acids with a single transmembrane domain. The extracellular region comprises six units of a fibronectin type III module, and part of this region of approximately 200 amino acids has features typical of a cytokine receptor family. A cDNA-expressed gp130 showed no binding property to IL-6 or several other cytokines. Although a transfectant with an IL-6-R cDNA expressed mainly low affinity IL-6 binding sites, an increase in high affinity binding sites was observed after cotransfection with a gp130 cDNA. This confirmed that a gp130 is involved in the formation of high affinity IL-6 binding sites. A cloned gp130 could associate with a complex of IL-6 and soluble IL-6-R and transduce the growth signal when expressed in a murine IL-3-dependent growth signal when expressed in a murine IL-3-dependent cell line.